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Trichome density in relation to volatiles emission and 1,8-Cineole synthase gene expression in Thymus albicans vegetative and reproductive organs

dc.contributor.authorMarques, N T.
dc.contributor.authorFilipe, Alexandra
dc.contributor.authorBarroso, Jose
dc.contributor.authorTrindade, Helena
dc.contributor.authorPower, Deborah
dc.contributor.authorFigueiredo, Ana Cristina
dc.contributor.authorPinto, Patricia IS
dc.date.accessioned2021-06-24T11:35:54Z
dc.date.available2021-06-24T11:35:54Z
dc.date.issued2020-03
dc.description.abstract1,8-Cineole is the main volatile produced by Thymus albicans Hoffmanns. & Link 1,8-cineole chemotype. To understand the contribution of distinct plant organs to the high 1,8-cineole production, trichome morphology and density, as well as emitted volatiles and transcriptional expression of the 1,8-cineole synthase (CIN) gene were determined separately for T. albicans leaves, bracts, calyx, corolla and inflorescences. Scanning electron microscopy (SEM) and stereoscope microscopy observations showed the highest peltate trichome density in leaves and bracts, significantly distinct from calyx and corolla. T. albicans volatiles were collected by solid phase micro extraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC/MS) and by GC for component identification and quantification, respectively. Of the 23 components identified, 1,8-cineole was the dominant volatile (57-93 %) in all T. albicans plant organs. The relative amounts of emitted volatiles clearly separated vegetative from reproductive organs. Gene expression of CIN was assigned to all organs analyzed and was consistent with the relatively high emission of 1,8-cineole in leaves and bracts. Further studies will be required to analyze monoterpenoid biosynthesis by each type of glandular trichome.
dc.description.sponsorshipCeratonia Project MONOTHYMUS, Universidade do Algarve, Portugal
dc.description.sponsorshipFCTPortuguese Foundation for Science and TechnologyEuropean Commission [SFRH/BPD/84033/2012]
dc.description.sponsorshipUniversity of Algarve
dc.description.sponsorshipCESAM [UID/AMB/50017-POCI-01-0145-FEDER-007638]
dc.description.sponsorshipFEDER, within the PT2020 Partnership Agreement
dc.description.sponsorship[FCT/UID/Multi/04326/2019/CCMAR]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1002/cbdv.201900669
dc.identifier.issn1612-1872
dc.identifier.urihttp://hdl.handle.net/10400.1/16570
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject1
dc.subject8-cineole
dc.subjectGlandular trichomes
dc.subjectLamiaceae
dc.subjectMonoterpenes
dc.subjectSPME
dc.subject.otherBiochemistry & Molecular Biology; Chemistry
dc.titleTrichome density in relation to volatiles emission and 1,8-Cineole synthase gene expression in Thymus albicans vegetative and reproductive organs
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.titleChemistry & Biodiversity
oaire.citation.volume17
person.familyNameTomás Marques
person.familyNamePower
person.familyNamePinto
person.givenNameNatália
person.givenNameDeborah Mary
person.givenNamePatricia IS
person.identifier643457
person.identifier.ciencia-id1510-CD2F-81CE
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.orcid0000-0002-0222-621X
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0001-7854-3898
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id10240774300
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4b1751aa-958b-4d68-ae97-ea628673ba00
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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