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Trichome density in relation to volatiles emission and 1,8-Cineole synthase gene expression in Thymus albicans vegetative and reproductive organs

2020-03Journal article Open Access
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dc.contributor.authorMarques, N T.
dc.contributor.authorFilipe, Alexandra
dc.contributor.authorPinto, Patricia
dc.contributor.authorBarroso, Jose
dc.contributor.authorTrindade, Helena
dc.contributor.authorPower, Deborah
dc.contributor.authorFigueiredo, Ana Cristina
dc.date.accessioned2021-06-24T11:35:54Z
dc.date.available2021-06-24T11:35:54Z
dc.date.issued2020-03
dc.description.abstract1,8-Cineole is the main volatile produced by Thymus albicans Hoffmanns. & Link 1,8-cineole chemotype. To understand the contribution of distinct plant organs to the high 1,8-cineole production, trichome morphology and density, as well as emitted volatiles and transcriptional expression of the 1,8-cineole synthase (CIN) gene were determined separately for T. albicans leaves, bracts, calyx, corolla and inflorescences. Scanning electron microscopy (SEM) and stereoscope microscopy observations showed the highest peltate trichome density in leaves and bracts, significantly distinct from calyx and corolla. T. albicans volatiles were collected by solid phase micro extraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC/MS) and by GC for component identification and quantification, respectively. Of the 23 components identified, 1,8-cineole was the dominant volatile (57-93 %) in all T. albicans plant organs. The relative amounts of emitted volatiles clearly separated vegetative from reproductive organs. Gene expression of CIN was assigned to all organs analyzed and was consistent with the relatively high emission of 1,8-cineole in leaves and bracts. Further studies will be required to analyze monoterpenoid biosynthesis by each type of glandular trichome.
dc.description.sponsorshipCeratonia Project MONOTHYMUS, Universidade do Algarve, Portugal
dc.description.sponsorshipFCTPortuguese Foundation for Science and TechnologyEuropean Commission [SFRH/BPD/84033/2012]
dc.description.sponsorshipUniversity of Algarve
dc.description.sponsorshipCESAM [UID/AMB/50017-POCI-01-0145-FEDER-007638]
dc.description.sponsorshipFEDER, within the PT2020 Partnership Agreement
dc.description.sponsorship[FCT/UID/Multi/04326/2019/CCMAR]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1002/cbdv.201900669
dc.identifier.issn1612-1872
dc.identifier.urihttp://hdl.handle.net/10400.1/16570
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject1
dc.subject8-cineole
dc.subjectGlandular trichomes
dc.subjectLamiaceae
dc.subjectMonoterpenes
dc.subjectSPME
dc.subject.otherBiochemistry & Molecular Biology; Chemistry
dc.titleTrichome density in relation to volatiles emission and 1,8-Cineole synthase gene expression in Thymus albicans vegetative and reproductive organs
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.titleChemistry & Biodiversity
oaire.citation.volume17
person.familyNameTomás Marques
person.familyNamePinto
person.familyNamePower
person.givenNameNatália
person.givenNamePatricia
person.givenNameDeborah Mary
person.identifier.ciencia-id1510-CD2F-81CE
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-0222-621X
person.identifier.orcid0000-0002-4532-6181
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4b1751aa-958b-4d68-ae97-ea628673ba00
relation.isAuthorOfPublication4acc3848-3327-419c-af05-53c1bbd40481
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery4acc3848-3327-419c-af05-53c1bbd40481

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