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Calcium mobilization from fish scales is mediated by parathyroid hormone related protein via the parathyroid hormone type 1 receptor

2005Journal article Restricted access
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dc.contributor.authorRotllant, J.
dc.contributor.authorRedruello, Begoña
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorFernandes, H.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-05-12T15:03:46Z
dc.date.available2014-05-12T15:03:46Z
dc.date.issued2005
dc.date.updated2014-05-09T08:55:18Z
dc.description.abstractThe scales of bony fish represent a significant reservoir of calcium but little is known about their contribution, as well as of bone, to calcium balance and how calcium deposition and mobilization are regulated in calcified tissues. In the present study we report the action of parathyroid hormone-related protein (PTHrP) on calcium mobilization from sea bream (Sparus auratus) scales in an in vitro bioassay. Ligand binding studies of piscine 125I-(1 – 35tyr)PTHrP to the membrane fraction of isolated sea bream scales revealed the existence of a single PTH receptor (PTHR) type. RT-PCR of fish scale cDNA using specific primers for two receptor types found in teleosts, PTH1R, and PTH3R, showed expression only of PTH1R. The signalling mechanisms mediating binding of the N-terminal amino acid region of PTHrP were investigated. A synthetic peptide (10 8 M) based on the N-terminal 1 – 34 amino acid residues of Fugu rubripes PTHrP strongly stimulated cAMP synthesis and [3 H]myo-inositol incorporation in sea bream scales. However, peptides (10 8 M) with N-terminal deletions, such as (2 – 34), (3 – 34) and (7 – 34)PTHrP, were defective in stimulating cAMP production but stimulated [3 H]myo-inositol incorporation. (1 – 34)PTHrP induced significant osteoclastic activity in scale tissue as indicated by its stimulation of tartrate-resistant acid phosphatase. In contrast, (7 – 34)PTHrP failed to stimulate the activity of this enzyme. This activity could also be abolished by the adenylyl cyclase inhibitor SQ-22536, but not by the phospholipase C inhibitor U-73122. The results of the study indicate that one mechanism through which N-terminal (1 – 34)PTHrP stimulates osteoclastic activity of sea bream scales, is through PTH1R and via the cAMP/AC intracellular signalling pathway. It appears, therefore, that fish scales can act as calcium stores and that (1 – 34)PTHrP regulates calcium mobilization from them; it remains to be established if this mechanism contributes to calcium homeostasis in vivo.por
dc.identifier.citationRotllant, J.; Redruello, B.; Guerreiro, P.M.; Fernandes, H.; Canario, A.V.M.; Power, D.M.Calcium mobilization from fish scales is mediated by parathyroid hormone related protein via the parathyroid hormone type 1 receptor, Regulatory Peptides, 132, 1-3, 33-40, 2005por
dc.identifier.issn01670115
dc.identifier.otherAUT: ACA00258; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/3869
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectPTHrPpor
dc.subjectTRACPpor
dc.subjectPTH1Rpor
dc.subjectOsteoclast activitypor
dc.subjectScales bioassaypor
dc.titleCalcium mobilization from fish scales is mediated by parathyroid hormone related protein via the parathyroid hormone type 1 receptorpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage40por
oaire.citation.startPage33por
oaire.citation.titleRegulatory Peptidespor
oaire.citation.volume132por
person.familyNameRedruello
person.familyNameGuerreiro da Costa Guerreiro
person.familyNameCanario
person.familyNamePower
person.givenNameBegoña
person.givenNamePedro Miguel
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifierA-2539-2009
person.identifier143624
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1787-5594
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryd0a8a148-98e7-4899-8fb0-0b8afa91e4a4

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