Repository logo
 
Publication

Transfection efficiency of chitosan and thiolated chitosan in retinal pigment epithelium cells: a comparative study

2013Journal article Restricted access
Simple item page
dc.contributor.authorSilva, Gabriela
dc.contributor.authorCosta, Ana M. Rosa da
dc.contributor.authorBitoque, Diogo
dc.contributor.authorOliveira, Ana V.
dc.contributor.authorSilva, Andreia P.
dc.date.accessioned2016-01-07T15:00:06Z
dc.date.available2016-01-07T15:00:06Z
dc.date.issued2013
dc.description.abstractGene therapy relies on efficient vector for a therapeutic effect. Efficient non-viral vectors are sought as an alternative to viral vectors. Chitosan, a cationic polymer, has been studied for its gene delivery potential. In this work, disulfide bond containing groups were covalently added to chitosan to improve the transfection efficiency. These bonds can be cleaved by cytoplasmic glutathione, thus, releasing the DNA load more efficiently. Chitosan and thiolated chitosan nanoparticles (NPs) were prepared in order to obtain a NH3 :PO ratio of 5:1 and characterized for plasmid DNA complexation and release efficiency. Cytotoxicity and gene delivery studies were carried out on retinal pigment epithelial cells. In this work, we show that chitosan was effectively modified to incorporate a disulfide bond. The transfection efficiency of chitosan and thiolated chitosan varied according to the cell line used, however, thiolation did not seem to significantly improve transfection efficiency. The apparent lack of improvement in transfection efficiency of the thiolated chitosan NPs is most likely due to its size increase and charge inversion relatively to chitosan. Therefore, for retinal cells, thiolated chitosan does not seem to constitute an efficient strategy for gene delivery.pt_PT
dc.identifier.citationJ Pharm Bioall Sci, (2013) 5(2) : 111-8pt_PT
dc.identifier.doihttp://dx.doi.org/10.4103/0975-7406.111823pt_PT
dc.identifier.issn0976-4879
dc.identifier.otherAUT: GAS02236; AMC01695;
dc.identifier.urihttp://hdl.handle.net/10400.1/7397
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMedknow Publicationspt_PT
dc.relationPIRG-GA-2009-249314pt_PT
dc.relationDEVELOPMENT OF NON-VIRAL VECTORS FOR GENE THERAPY FOR PATHOLOGIES OF THE RETINA
dc.relation.publisherversionhttp://www.jpbsonline.org/article.asp?issn=0975-7406;year=2013;volume=5;issue=2;spage=111;epage=118;aulast=Oliveirapt_PT
dc.subjectChitosanpt_PT
dc.subjectGene therapypt_PT
dc.subjectNon-viral vectorspt_PT
dc.subjectThiolationpt_PT
dc.subjectTransfection efficiencypt_PT
dc.titleTransfection efficiency of chitosan and thiolated chitosan in retinal pigment epithelium cells: a comparative studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT OF NON-VIRAL VECTORS FOR GENE THERAPY FOR PATHOLOGIES OF THE RETINA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BEB%2F098475%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70318%2F2010/PT
oaire.citation.endPage118pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage111pt_PT
oaire.citation.titleJournal of Pharmacy and BioAllied Sciencespt_PT
oaire.citation.volume5pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameSilva
person.familyNameRosa da Costa
person.familyNameBitoque
person.givenNameGabriela
person.givenNameAna M
person.givenNameDiogo
person.identifier305029
person.identifier2140108
person.identifier.ciencia-idDC1E-F164-FDD9
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.ciencia-idD81C-6457-7F28
person.identifier.orcid0000-0001-5946-1918
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0002-6536-6578
person.identifier.ridF-4409-2012
person.identifier.ridE-2165-2012
person.identifier.scopus-author-id7101647767
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id55755464000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6e56eaba-fa8c-4d24-9f54-008c266c1c4b
relation.isAuthorOfPublication0ca8c16f-6881-4662-bafc-cc51c5ce8d88
relation.isAuthorOfPublication82f1e5e9-2f1d-48dc-92f9-30e7d4f97650
relation.isAuthorOfPublication.latestForDiscovery82f1e5e9-2f1d-48dc-92f9-30e7d4f97650
relation.isProjectOfPublication610cf07c-9c35-43ca-933b-27e8122a5fcd
relation.isProjectOfPublicationea46c108-e292-4eb0-abb6-39add8a55215
relation.isProjectOfPublication.latestForDiscovery610cf07c-9c35-43ca-933b-27e8122a5fcd

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Oliveira, 2013.pdf
Size:
420.45 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCT2-Artigos (em revistas ou actas indexadas)