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Involvement of metallothionein in Zn accumulation and elimination strategies in Ruditapes decussatus

2006Journal article Restricted access
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dc.contributor.authorSerafim, Angela
dc.contributor.authorBebianno, Maria João
dc.date.accessioned2020-05-07T09:09:51Z
dc.date.available2020-05-07T09:09:51Z
dc.date.issued2006
dc.description.abstractThe aim of this study was to identify the involvement of metallothionein (MT) in Zn accumulation and elimination strategies in different tissues (gills, digestive gland, and remaining tissues) of Ruditapes decussatus. Clams were exposed to two Zn concentrations (100 and 1000 microg/L) for 40 days, followed by a depuration period of 50 days. The accumulation and elimination processes were complemented by subcellular Zn distribution, to determine the affinity of this element for the different cellular partitions. Subsequently, the involvement of MT was followed. Zinc concentrations in the tissues of R. decussatus exposed to 100 microg/L was partially regulated, whereas in those exposed to 1000 microg/L, Zn was accumulated (exponentially and linearly) throughout the exposure period. The greatest amount of Zn is associated to the insoluble fraction; however, in the highest Zn exposure, approximately 30% is in the thermostable fraction, where MT occurs. Gel filtration chromatography confirmed that the Zn ions accumulated in this fraction were bound to MT. This protein is actively involved in the elimination of this metal, through the rapid degradation of the Zn-MT complex because MT and Zn are turning over simultaneously. Therefore, MT participates in Zn homeostasis in R. decussatus when Zn exposure is low. When Zn accumulation increases, the role of this protein changes from a constitutive function to the detoxification of the excess of Zn ions.pt_PT
dc.description.sponsorshipPortuguese Foundation of Science and Technology grant (CIÊNCIA/BD/2541/93)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00244-005-0258-6pt_PT
dc.identifier.issn0090-4341
dc.identifier.urihttp://hdl.handle.net/10400.1/13850
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00244-005-0258-6pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnalysis of variancept_PT
dc.subjectAnimalspt_PT
dc.subjectBivalviapt_PT
dc.subjectGel chromatographypt_PT
dc.subjectGastric mucosapt_PT
dc.subjectGillspt_PT
dc.subjectMetallothioneinpt_PT
dc.subjectTissue distributionpt_PT
dc.subjectZincpt_PT
dc.titleInvolvement of metallothionein in Zn accumulation and elimination strategies in Ruditapes decussatuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage199pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage189pt_PT
oaire.citation.titleArchives of Environmental Contamination and Toxicologypt_PT
oaire.citation.volume52pt_PT
person.familyNamePereira Serafim
person.familyNameBebianno
person.givenNameMaria Angela
person.givenNameMaria
person.identifier.ciencia-id9C18-090A-5E3D
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0002-1165-0645
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0e42fc03-7052-438d-952b-b3caa5bdc36a
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery0e42fc03-7052-438d-952b-b3caa5bdc36a

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