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Sex steroids and metabolic responses in mussels Mytilus galloprovincialis exposed to drospirenone

2017-09Journal article Restricted access
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dc.contributor.authorCappello, Tiziana
dc.contributor.authorFernandes, Denise
dc.contributor.authorMaisano, Maria
dc.contributor.authorCasano, Andrea
dc.contributor.authorBonastre, Marta
dc.contributor.authorBebianno, Maria João
dc.contributor.authorMauceri, Angela
dc.contributor.authorFasulo, Salvatore
dc.contributor.authorPorte, Cinta
dc.date.accessioned2019-11-20T15:07:25Z
dc.date.available2019-11-20T15:07:25Z
dc.date.issued2017-09
dc.description.abstractDrospirenone (DRO) is a synthetic progestin derived from 17 alpha-spironolactone with a pharmacological mechanism of action similar to progesterone. Despite its wide use as pharmaceutical and consequent continuous release into the aquatic environment, DRO effects have been poorly investigated on aquatic biota. In order to unravel the toxicity mechanisms of DRO, mussels Mytilus galloprovincialis were exposed for 7 days to different concentrations of DRO, namely 20 ng/L (Low; L), 200 ng/L (Medium; M), 2000 ng/L (High; H) and 10 mu g/L (Super High; SH) nominal doses. Following exposure, no significant effect was observed on gonad maturation of treated and untreated mussels. The levels of progesterone (P4) and testosterone (T) were measured in mantle/gonad tissues and no significant alteration detected after exposure. However, the application of a protonic nuclear magnetic resonance (H-1 NMR)-based metabolomics approach enabled a comprehensive assessment of DRO effects in mussels. Specifically, H-1 NMR metabolic fingerprints of digestive glands of DRO treated mussel groups were clearly separated from each other and from controls through a principal component analysis (PCA). Moreover, a number of metabolites involved in different metabolic pathways were found to significantly change in DRO-exposed mussels compared to control, suggesting the occurrence of alterations in energy metabolism, amino acids metabolism, and glycerophospholipid metabolism. Overall, despite no changes in gonad maturation and steroids levels were recorded in mussels after DRO exposure, the metabolomics approach demonstrated its effectiveness and high sensitivity in elucidating DRO-induced metabolic disturbances in marine mussels, and thus its usefulness in the environmental risk assessment of pharmaceuticals.
dc.description.sponsorshipSpanish National Plan for Research [CGL2011-24538]
dc.identifier.doi10.1016/j.ecoenv.2017.05.031
dc.identifier.issn0147-6513
dc.identifier.issn1090-2414
dc.identifier.urihttp://hdl.handle.net/10400.1/13029
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectNmr-based metabolomics
dc.subjectZebrafish danio-rerio
dc.subjectEnvironmentally relevant concentrations
dc.subjectMediterranean Coastal Lagoon
dc.subjectFish liza-aurata
dc.subjectH-1-Nmr metabolomics
dc.subjectOxidative stress
dc.subjectProgesterone
dc.subjectBioaccumulation
dc.subjectMechanisms
dc.titleSex steroids and metabolic responses in mussels Mytilus galloprovincialis exposed to drospirenone
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage172
oaire.citation.startPage166
oaire.citation.titleEcotoxicology and Environmental Safety
oaire.citation.volume143
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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