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ESSA1 embryonic stem like cells from gilthead seabream: a new tool to study mesenchymal cell lineage differentiation in fish

dc.contributor.authorParameswaran, V.
dc.contributor.authorLaizé, Vincent
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-05T12:53:10Z
dc.date.available2014-06-05T12:53:10Z
dc.date.issued2012
dc.date.updated2014-05-30T09:59:29Z
dc.description.abstractEmbryonic stem (ES) cells are a promising tool for generation of transgenic animals and an ideal experimental model for in vitro studies of embryonic cell development, differentiation and gene manipulation. Here we report the development and initial characterization of a pluripotent embryonic stem like cell line, designated as ESSA1, derived from blastula stage embryos of the gilthead seabream (Sparus aurata, L). ESSA1 cells are cultured in Leibovitz’s L-15 medium supplemented with 5% fetal bovine serum and, unlike other ES cells, without a feeder layer. They have a round or polygonal morphology, grow exponentially in culture and form dense colonies. ESSA1 cells also exhibit intense alkaline phosphatase activity, normal karyotype and are positive for stage-specific embryonic antigen-1 (SSEA1) and octamer-binding transcription factor 4 (Oct4) markers for up to 30 passages. Upon treatment with all-trans retinoic acid, ESSA1 cells differentiate into neuron-like, oligodendritic, myocyte and melanocyte cells; they can also form embryoid bodies when seeded in bacteriological plates, a characteristic usually associated with pluripotency. The capacity of ESSA1 cells to differentiate into osteoblastic, chondroblastic or osteoclastic cell lineages and to produce a mineralized extracellular matrix in vitro was demonstrated through histochemical techniques and further confirmed by immunocytochemistry using lineage-specific markers. Furthermore, ESSA1 cells can be used to produce chimera, where they contribute to the development of a variety of tissues including the trunk and gut of zebrafish embryos and fry. Thus, ESSA1 cells represent a promising model for investigating bonelineage cell differentiation in fish and also highlight the potential of piscine stem cell research.por
dc.identifier.citationParameswaran, V.; Laizé, V.; Gavaia, P.J.; Leonor Cancela, M. ESSA1 embryonic stem like cells from gilthead seabream: A new tool to study mesenchymal cell lineage differentiation in fish, Differentiation, 84, 3, 240-251, 2012.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.diff.2012.07.004
dc.identifier.issn0301-4681
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4194
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectFish-derived ES cellspor
dc.subjectGilthead seabream Sparus auratapor
dc.subjectPluripotencypor
dc.subjectEmbryoid bodypor
dc.subjectBone cell typepor
dc.subjectCell differentiationpor
dc.titleESSA1 embryonic stem like cells from gilthead seabream: a new tool to study mesenchymal cell lineage differentiation in fishpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage251por
oaire.citation.issue3por
oaire.citation.startPage240por
oaire.citation.titleDifferentiationpor
oaire.citation.volume84por
person.familyNameLaizé
person.familyNameGavaia
person.familyNameCancela
person.givenNameVincent
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id6602982778
person.identifier.scopus-author-id6507104377
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscovery9dca2139-21a4-4d59-aaf7-531f1033a58e

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