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An expressed sequence tag analysis of the intertidal brown seaweeds Fucus serratus (L.) and F. vesiculosus (L.) (Heterokontophyta, Phaeophyceae) in response to abiotic stressors

2010Journal article Restricted access
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dc.contributor.authorPearson, G. A.
dc.contributor.authorHoarau, G.
dc.contributor.authorLago-Lestón, Asunción
dc.contributor.authorCoyer, J. A.
dc.contributor.authorKube, M.
dc.contributor.authorReinhardt, Richard
dc.contributor.authorHenckel, K.
dc.contributor.authorSerrão, Ester
dc.contributor.authorCorre, E.
dc.contributor.authorOlsen, J. L.
dc.date.accessioned2014-06-03T13:22:35Z
dc.date.available2014-06-03T13:22:35Z
dc.date.issued2010
dc.date.updated2014-05-21T13:13:04Z
dc.description.abstractIn order to aid gene discovery and uncover genes responding to abiotic stressors in stress-tolerant brown algae of the genus Fucus, expressed sequence tags (ESTs) were studied in two species, Fucus serratus and Fucus vesiculosus. Clustering of over 12,000 ESTs from three libraries for heat shock/recovery and desiccation/rehydration resulted in identification of 2,503, 1,290, and 2,409 unigenes from heat-shocked F. serratus, desiccated F. serratus, and desiccated F. vesiculosus, respectively. Low overall annotation rates (18–31%) were strongly associated with the presence of long 3′ untranslated regions in Fucus transcripts, as shown by analyses of predicted protein-coding sequence in annotated and nonannotated tentative consensus sequences. Posttranslational modification genes were overrepresented in the heat shock/recovery library, including many chaperones, the most abundant of which were a family of small heat shock protein transcripts, Hsp90 and Hsp70 members. Transcripts of LI818-like light-harvesting genes implicated in photoprotection were also expressed during heat shock in high light. The expression of several heat-shock-responsive genes was confirmed by quantitative reverse transcription polymerase chain reaction. However, candidate genes were notably absent from both desiccation/rehydration libraries, while the responses of the two species to desiccation were divergent, perhaps reflecting the species-specific physiological differences in stress tolerance previously established. Desiccation-tolerant F. vesiculosus overexpressed at least 17 ribosomal protein genes and two ubiquitinribosomal protein fusion genes, suggesting that ribosome function and/or biogenesis are important during cycles of rapid desiccation and rehydration in the intertidal zone and possibly indicate parallels with other poikilohydric organisms such as desiccation-tolerant bryophytes.por
dc.identifier.citationPearson, G.A.; Hoarau, G.; Lago-Leston, A.; Coyer, J.A.; Kube, M.; Reinhardt, R.; Henckel, K.; Serrão, E.T.A.; Corre, E.; Olsen, J.L. An expressed sequence tag analysis of the intertidal brown seaweeds Fucus serratus (L.) and F. vesiculosus (L.) (Heterokontophyta, Phaeophyceae) in response to abiotic stressors, Marine Biotechnology, 12, 2, 195-213, 2010.por
dc.identifier.doihttp://dx.doi.org/10.1007/s10126-009-9208-z
dc.identifier.issn1436-2228
dc.identifier.otherAUT: ESE00527;
dc.identifier.urihttp://hdl.handle.net/10400.1/4162
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectAbiotic stress responsepor
dc.subjectBrown algapor
dc.subjectDesiccationpor
dc.subjectEST librarypor
dc.subjectEcological genomicspor
dc.subjectFucus serratuspor
dc.subjectFucus vesiculosuspor
dc.subjectGene expression profilepor
dc.subjectHeat shockpor
dc.titleAn expressed sequence tag analysis of the intertidal brown seaweeds Fucus serratus (L.) and F. vesiculosus (L.) (Heterokontophyta, Phaeophyceae) in response to abiotic stressorspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage213por
oaire.citation.issue2por
oaire.citation.startPage195por
oaire.citation.titleMarine Biotechnologypor
oaire.citation.volume12por
person.familyNamePearson
person.familyNameLago-Lestón
person.familyNameSerrao
person.givenNameGareth Anthony
person.givenNameAsunción
person.givenNameEster A.
person.identifier113536
person.identifierC-6686-2012
person.identifier.ciencia-id3315-9919-1A52
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.orcid0000-0002-0768-464X
person.identifier.orcid0000-0003-4361-7732
person.identifier.orcid0000-0003-1316-658X
person.identifier.ridJ-3911-2013
person.identifier.scopus-author-id55916875600
person.identifier.scopus-author-id57193972525
person.identifier.scopus-author-id7004093604
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication0a10c448-c7f5-4c3b-9488-3917c707e35e
relation.isAuthorOfPublicationcf3375ea-836f-4de2-9cd6-fde518b26838
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublication.latestForDiscovery45ccfe90-155c-4d6f-9e86-8f0fd064005f

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