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Galanin isoforms by alternative splicing: structure, expression, and immunohistochemical location in the gonads of European sea bass

2021Journal article Restricted access
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dc.contributor.authorMartins, Rute
dc.contributor.authorSousa, Carmen
dc.contributor.authorAndrade, André
dc.contributor.authorMolés, Gregorio
dc.contributor.authorZanuy, Silvia
dc.contributor.authorGómez, Ana
dc.contributor.authorCanario, Adelino
dc.contributor.authorPinto, Patrícia
dc.date.accessioned2021-04-12T14:34:34Z
dc.date.available2022-05-01T00:30:15Z
dc.date.issued2021
dc.description.abstractGalanin (Gal) is a neuropeptide with multiple functions that is widely expressed in the central and peripheral nervous systems of vertebrates. Anatomical and functional evidence suggests a possible role in regulating reproduction in fishes. To test this possibility, we have isolated and characterized two gal alternative transcripts in European sea bass (Dicentrarchus labrax) that encode two prepropeptides, respectively of 29 (gal_MT853221) and 53 (gal_MT853222) amino acids. The two gal transcripts are highly expressed in brain, pituitary and gonads, and appear to be differentially regulated in males and females. In males, gal_MT853222 in the hypothalamus and gal_MT853221 in the pituitary were downregulated with the progression of spermatogenesis (stages I-III). Both transcripts are downregulated in testicles of 1-year (precocious) and 2-year spermiating males compared to immature fish of the same age. Gal peptides and receptors are expressed throughout ovarian development in the hypothalamic-pituitary-gonadal (HPG) axis of females. In the testis, immunoreactive Gal-29 and Gal-53 peptides were detected in blood vessels and Leydig cells during the spermatogenesis stages I-III but Gal immunostaining was barely undetected in more advanced stages. In the ovary, both peptides localized in interstitial cells and blood vessels and in theca cells surrounding the maturing oocytes. The immunolocalization of galanin in Leydig and theca cells suggests a possible role in steroid production regulation. The different pattern of gal expression and Gal localization in the testis and ovary may suggest the possibility that androgens and estrogens may also regulate Gal gene transcription and translation. Altogether, this study showed evidence for the possible involvement of locally produced Gal in gametogenesis and that its production is differentially regulated in male and female gonads.pt_PT
dc.description.sponsorshipFCT (Foundation of Science and Technology, I.P.) UIDB/04326/20207 PTDC/MAR-BIO/3890/2012; European Union Seventh Framework Programme AQUAEXCEL 262336, 318 Spanish MINECO: AGL2015-67477-C2-1-R Consejo Superior de Investigaciones Cientificas (CSIC):201640E073 Portuguese Foundation for Science and Technology-European Commission: DL 57/2016/CP1361/CT0021 DL57/2016/CP6321/CT0015pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ygcen.2021.113730pt_PT
dc.identifier.issn0016-6480
dc.identifier.urihttp://hdl.handle.net/10400.1/15368
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectSea basspt_PT
dc.subjectSpermatogenesispt_PT
dc.subjectGalaninpt_PT
dc.subjectOogenesispt_PT
dc.subjectGalanin receptorspt_PT
dc.subjectFSHpt_PT
dc.subjectLHpt_PT
dc.titleGalanin isoforms by alternative splicing: structure, expression, and immunohistochemical location in the gonads of European sea basspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-BIO%2F3890%2F2012/PT
oaire.citation.startPage113730pt_PT
oaire.citation.titleGeneral and Comparative Endocrinologypt_PT
oaire.citation.volume305pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameMartins
person.familyNameVieira de Sousa
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNameCanario
person.familyNamePinto
person.givenNameRute Sofia Tavares
person.givenNameCármen Sofia
person.givenNameAndré
person.givenNameAdelino
person.givenNamePatrícia
person.identifier143624
person.identifier.ciencia-idA418-0899-1BD9
person.identifier.ciencia-idE511-2F93-F25D
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id1215-D615-204C
person.identifier.orcid0000-0001-5804-2981
person.identifier.orcid0000-0002-3483-1932
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0002-3153-2830
person.identifier.ridC-7942-2009
person.identifier.ridC-3197-2018
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id57200566502
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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