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Exploration of the types of rarity in the Arctic Ocean from the perspective of multiple methodologies

2021Journal article Restricted access
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dc.contributor.authorPascoal, Francisco
dc.contributor.authorCosta, Rodrigo
dc.contributor.authorAssmy, Philipp
dc.contributor.authorDuarte, Pedro
dc.contributor.authorMagalhães, Catarina
dc.date.accessioned2021-09-30T08:35:01Z
dc.date.available2021-09-30T08:35:01Z
dc.date.issued2021
dc.description.abstractThe Arctic Ocean is facing rapid environmental changes with cascading effects on the entire Arctic marine ecosystem. However, we have a limited understanding of the consequences such changes have on bacteria and archaea (prokaryotes) at the base of the marine food web. In this study, we show how the prokaryotic rare biosphere behaves over a range of highly heterogeneous environmental conditions using 16S rRNA gene reads from amplicon and metagenome sequencing data from seawater samples collected during the Norwegian young sea ICE expedition between late winter and early summer. The prokaryotic rare biosphere was analyzed using different approaches: amplicon sequence variants and operational taxonomic units from the 16S rRNA gene amplicons and operational taxonomic units from the 16S rRNA genes of the metagenomes. We found that prokaryotic rare biosphere communities are specific to certain water masses, and that the majority of the rare taxa identified were always rare and disappeared in at least one sample under changing conditions, suggesting their high sensitivity to environmental heterogeneity. In addition, our methodological comparison revealed a good performance of 16S rRNA gene amplicon sequencing in describing rare biosphere patterns, while the metagenome-derived data were better to capture a significant diversity of so-far uncultivated rare taxa. Our analysis on the dynamics of the rare prokaryotic biosphere, by combining different methodological approaches, improves the description of the types of rarity predicted from Community Assembly theory in the Arctic Ocean.pt_PT
dc.description.sponsorshipUIDB/04423/2020; UIDP/04565/2020; Programa Operacional Regional de Lisboa 007317pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00248-021-01821-9pt_PT
dc.identifier.issn0095-3628
dc.identifier.urihttp://hdl.handle.net/10400.1/17173
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute for Bioengineering and Biosciences
dc.subjectTypes of raritypt_PT
dc.subjectASVs vs. OTUspt_PT
dc.subjectArctic Ocean microbiomept_PT
dc.subjectMarine microbial ecologypt_PT
dc.subjectPolar microbial ecologypt_PT
dc.subjectMolecular ecologypt_PT
dc.titleExploration of the types of rarity in the Arctic Ocean from the perspective of multiple methodologiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.citation.titleMicrobial Ecologypt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameda Silva Costa
person.givenNameRodrigo
person.identifier115920
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id7203063627
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery4495127c-16f2-4231-9fc1-b471c661036b
relation.isProjectOfPublication2918f082-1ced-47c5-a6ea-eff9b8eaca1f
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