Repository logo
 
Publication

Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro

2005Journal article Open access
Simple item page
dc.contributor.authorRotllant, J.
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorAnjos, Liliana
dc.contributor.authorRedruello, Begoña
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-05-17T10:51:50Z
dc.date.available2014-05-17T10:51:50Z
dc.date.issued2005
dc.date.updated2014-05-13T10:08:55Z
dc.description.abstractThe mode of action of PTHrP in the regulation of sea bream (Sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system. Piscine (1–34)PTHrP (10−6–10−11 m) stimulated cortisol production in a dose-dependent manner. The ED50 of (1–34)PTHrP was 2.8 times higher than that of (1–39)ACTH, and maximum increase in cortisol production in response to 10−8 m of (1–34)PTHrP was approximately 7-fold lower than for 10−8 m of (1–39)ACTH. In contrast to (1–34)PTHrP, piscine (10–20)PTHrP, (79–93)PTHrP, and (100–125)PTHrP (10−9–10−7 m) did not stimulate cortisol production. The effect of piscine (1–34)PTHrP on cortisol production was abolished by N-terminal peptides in which the first amino acid (Ser) was absent and by simultaneous addition of inhibitors of the adenylyl cyclase-protein kinase A and phospholipase C-protein kinase C intracellular pathways but not by each separately. The PTHrP-induced signal transduction was further investigated by measurements of cAMP production and [H3]myo-inositol incorporation in an interrenal cell suspension. Piscine (1–34)PTHrP increased cAMP and total inositol phosphate accumulation, which is indicative that the mechanism of action of PTHrP in interrenal tissue involves the activation of both the adenylyl cyclase-cAMP and phospholipase C-inositol phosphate signaling pathways. These results, together with the expression of mRNA for PTHrP and for PTH receptor (PTHR) type 1 and PTHR type 3 receptors in sea bream interrenal tissue, suggest a specific paracrine or autocrine steroidogenic action of PTHrP mediated by the PTHRs.por
dc.identifier.citationRotllant, J.; Guerreiro, P. M.; Anjos, L.; Redruello, B.; Canario, A. V. M.; Power, D. M.Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro, Endocrinology, 146, 1, 71-76, 2005.por
dc.identifier.doihttp://dx.doi.org/10.1210/en.2004-0644
dc.identifier.issn0013-7227
dc.identifier.otherAUT: ACA00258; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/3994
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherEndocrine Societypor
dc.relation.publisherversionhttp://press.endocrine.org/doi/abs/10.1210/en.2004-0644por
dc.titleStimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitropor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage76por
oaire.citation.issue1
oaire.citation.startPage71por
oaire.citation.titleEndocrinologypor
oaire.citation.volume146por
person.familyNameGuerreiro da Costa Guerreiro
person.familyNameAnjos Guerreiro
person.familyNameRedruello
person.familyNameCanario
person.familyNamePower
person.givenNamePedro Miguel
person.givenNameLiliana Isabel Tomé
person.givenNameBegoña
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifierA-2539-2009
person.identifierAAK-3800-2020
person.identifier143624
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0003-1787-5594
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd0a8a148-98e7-4899-8fb0-0b8afa91e4a4
relation.isAuthorOfPublication721b5dca-ad42-40f8-9731-517eb5db009e
relation.isAuthorOfPublication67798ebb-16f5-4974-8d52-5158ddf9fe53
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Stimulation of Cortisol Release by the N Terminus of Teleost Parathyroid Hormone-Related Protein in Interrena.pdf
Size:
378.07 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)