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Identification of estrogen-responsive genes in the testis of sea bream (Sparus auratus) using suppression subtractive hybridization

2006Journal article Restricted access
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dc.contributor.authorPinto, Patricia IS
dc.contributor.authorTeodósio, H. R.
dc.contributor.authorGalay-Burgos, M.
dc.contributor.authorPower, Deborah
dc.contributor.authorSweeney, Glen E.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-15T09:43:36Z
dc.date.available2014-05-15T09:43:36Z
dc.date.issued2006
dc.date.updated2014-05-13T09:32:00Z
dc.description.abstractThere is growing evidence that estrogens play important roles in both normal and xenoestrogen disrupted testis physiology. However, the mechanisms and signaling pathways involved, in particular in fish, are largely unknown. We have used suppression subtractive hybridization to isolate 152 candidate estrogen-responsive genes in the testis of male estradiol (E2)-treated sea bream (Sparus aurata). The E2 up-regulation of some of the genes (e.g., choriogenin L and H, vitellogeni I and II, apolipoprotein A-I, fibrinogen b and g, and thyroid receptor interacting protein 4) was confirmed by reverse transcriptase polymerase chain reaction in fish treated with 0.1–10 mg/kg E2. Many of these genes are typical E2-induced genes in liver, and this is the first report of its up regulation with E2 in testis. Moreover, low levels of expression were also found for nontreated fish. Hepatic differential expression for these genes was also confirmed, although, contrary to testis, fibrinogen b, and g were downregulated. The possible significance of these findings in normal testis physiology and in endocrine disruption is discussed.por
dc.identifier.citationPinto, P. I. S.; Teodosio, H. R.; Galay-Burgos, M.; Power, D. M.; Sweeney, G. E.; Canario, A. V. M. Identification of estrogen-responsive genes in the testis of sea bream (Sparus auratus) using suppression subtractive hybridization, Molecular Reproduction and Development, 73, 3, 318-329, 2006.por
dc.identifier.doihttp://dx.doi.org/10.1002/mrd.20402
dc.identifier.issn1040-452X
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3947
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.subjectEstradiolpor
dc.subjectVitellogeninpor
dc.subjectChoriogeninpor
dc.subjectEgg shell proteinspor
dc.subjectReproductionpor
dc.subjectEndocrine disruptionpor
dc.titleIdentification of estrogen-responsive genes in the testis of sea bream (Sparus auratus) using suppression subtractive hybridizationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage329por
oaire.citation.issue3por
oaire.citation.startPage318por
oaire.citation.titleMolecular Reproduction and Developmentpor
oaire.citation.volume73por
person.familyNamePinto
person.familyNamePower
person.familyNameCanario
person.givenNamePatricia IS
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier643457
person.identifier143624
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-3817-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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