Logo do repositório
 
Publicação

Parathyroid hormone-related protein-stanniocalcin antagonism in regulation of bicarbonate secretion and calcium precipitation in a marine fish intestine

2010Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorFuentes, J.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-10-24T13:24:42Z
dc.date.available2014-10-24T13:24:42Z
dc.date.issued2010
dc.description.abstractParathyroid hormone-related protein-stanniocalcin antagonism in regulation of bicarbonate secretion and calcium precipitation in a marine fish intestine. Am J Physiol Regul Integr Comp Physiol 299: R150–R158, 2010. First published April 21, 2010; doi:10.1152/ajpregu.00378.2009.—Bicarbonate secretion in the intestine (duodenum) of marine fish has been suggested to play a major role in regulation of calcium availability for uptake. However, while the end process may lead to carbonate precipitation, regulation of transport of calcium and/or bicarbonate may actually result in fine-tuning of calcium availability for transport. To test this hypothesis, sea bream (Sparus auratus) duodenal preparations were mounted in Ussing-type chambers and the effect of parathyroid hormone-related protein (PTHrP) and stanniocalcin 1 (STC 1) on the control of intestinal bicarbonate secretion and calcium transport was analyzed. As expected, PTHrP increased net calcium uptake, as a result of an increase of calcium uptake without changes in calcium efflux. In contrast, purified sea bream STC 1 caused a minor decrease of calcium uptake and a two- to threefold increase in calcium efflux. As a result, STC 1 was able to invert the calcium flux from net calcium uptake to net calcium loss, which is in keeping with its known actions as a hypocalcemic factor. Furthermore, both PTHrP and STC 1 regulate intestinal bicarbonate secretion. PTHrP increased calcium uptake and simultaneously reduced the single factor that induces calcium precipitation, bicarbonate secretion. In contrast, STC 1, while reversing the calcium net flux to make it secretory, promoted intestinal bicarbonate secretion, both actions directed to decrease the calcium gradient across the epithelium and promote immobilization in the form of bicarbonate in the intestinal lumen. Together our results provide robust evidence to support an antagonistic action of PTHrP and STC 1 in the fine control of movements of both calcium and bicarbonate in the intestine of seawater fish.por
dc.description.sponsorshipThis work was supported by Ministry of Science and Higher Education and European Social Funds through the Portuguese National Science Foundation Projects POCTI/CVT/55683/2004 and TDC/MAR/104008/2008 to J. Fuentes.por
dc.identifier.citationJuan Fuentes, Deborah M. Power and Adelino V. M. Canário, "Parathyroid hormone-related protein-stanniocalcin antagonism in regulation of bicarbonate secretion and calcium precipitation in a marine fish intestine" in Am J Physiol Regul Integr Comp Physiol 299:R150-R158, 2010.por
dc.identifier.doihttp://dx.doi.org/10.1152/ajpregu.00378.2009
dc.identifier.issn0363-6119
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/5491
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Physiological Societypor
dc.relation.publisherversionhttp://ajpregu.physiology.org/content/299/1/R150por
dc.subjectIon regulationpor
dc.subjectSea waterpor
dc.subjectSea breampor
dc.titleParathyroid hormone-related protein-stanniocalcin antagonism in regulation of bicarbonate secretion and calcium precipitation in a marine fish intestinepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPageR158por
oaire.citation.issue1por
oaire.citation.startPageR150por
oaire.citation.titleAmerican Journal of Physiology - Regulatory, Integrative and Comparative Physiologypor
oaire.citation.volume299por
person.familyNameFuentes
person.familyNamePower
person.familyNameCanario
person.givenNameJuan
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier392998
person.identifier143624
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Parathyroid hormone-related protein-stanniocalcin antagonism in regulation of bicarbonate secretion and calcium precipitation in a marine fish intestine.pdf
Tamanho:
852.4 KB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
3.49 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CCM2-Artigos (em revistas ou actas indexadas)