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A cDNA for European sea bass (Dicentrachus labrax) 11 beta-hydroxylase: Gene expression during the thermosensitive period and gonadogenesis

2007Journal article Restricted access
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dc.contributor.authorSocorro, S.
dc.contributor.authorMartins, Rute S. T.
dc.contributor.authorDeloffre, Laurence A. M.
dc.contributor.authorMylonas, Constantinos C.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-09T09:39:56Z
dc.date.available2014-05-09T09:39:56Z
dc.date.issued2007
dc.date.updated2014-05-08T11:34:15Z
dc.description.abstractSteroid P450 11 -hydroxylase, encoded by the CYP11B gene, is a key mitochondrial enzyme for the production of 11-oxygenated androgens, which have been shown to be potent masculinising steroids in several Wsh species. In this study we have isolated a CYP11B cDNA of 1903 base pairs from the testis of European sea bass (Dicentrarchus labrax) encoding a predicted protein of 552 amino acids. The amino acid identities to other vertebrate 11 -hydroxylase proteins ranged from 66% to 72% to other Wsh; 45% to amphibian and 35–39% to mammalian. Southern blot indicated that a single CYP11B gene is present. Northern blot analysis detected two transcripts in testis and head kidney, one of the same size of the isolated clone and the other of 3.9 kb. Reverse transcriptase-polymerase chain reaction showed abundant mRNA expression only in testis and head kidney, being residual in a range of other tissues. Expression of CYP11B and CYP19A (which encodes for ovarian aromatase) was detected from at least 4 days post-hatching and did not appear to be aVected by rearing temperature (15 and 20 °C) during the Wrst 60 days, a period in which high temperatures promote masculinisation in European sea bass. Throughout, gonadogenesis (60–300 dph), a highly dimorphic pattern of CYP11B expression was consistent with a role of this gene in testicular development.por
dc.identifier.citationSocorro, Silvia; Martins, Rute S.T.; Deloffre, Laurence A.M.; Mylonas, Constantinos C.; Canario, Adelino V. M. A cDNA for European sea bass (Dicentrachus labrax) 11 beta-hydroxylase: Gene expression during the thermosensitive period and gonadogenesis, General and Comparative Endocrinology, 150, 1, 164-173, 2007.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2006.07.018
dc.identifier.issn0016-6480
dc.identifier.otherAUT: ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3817
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectCYP11Bpor
dc.subjectDicentrarchus labraxpor
dc.subjectAromatasepor
dc.subject11 -Hydroxylasepor
dc.subjectTemperaturepor
dc.subjectSex diVerentiationpor
dc.subjectAndrogenspor
dc.subjectCYP19Apor
dc.titleA cDNA for European sea bass (Dicentrachus labrax) 11 beta-hydroxylase: Gene expression during the thermosensitive period and gonadogenesispor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage173por
oaire.citation.issue1por
oaire.citation.startPage164por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume150por
person.familyNameCanario
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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