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Genetic diversity of wild populations of Tuberaria major (Cistaceae), an endangered species endemic to the Algarve region (Portugal), using ISSR markers

dc.contributor.authorTrindade, Helena
dc.contributor.authorSena, Inês
dc.contributor.authorGonçalves, Sandra
dc.contributor.authorRomano, Anabela
dc.date.accessioned2013-03-15T11:32:08Z
dc.date.available2013-03-15T11:32:08Z
dc.date.issued2012
dc.description.abstractTuberaria major (Willk.) P. Silva & Rozeira (Cistaceae) is an endangered species endemic to the Algarve region (Portugal). Due to the increasing urban pressure, natural populations are now reduced to small nuclei located in the center of the region. Genetic variation within and among three populations of this species was evaluated using inter-simple sequence repeat (ISSR) markers. A total of eight primers generated 128 well defined bands, with an average of 16 bands per primer. At the population level, the percentage of polymorphic bands ranged from 55 to 73%, while at the species level this value increased to 98%. The population showing the highest genetic variability was Olhão and the lowest was Gambelas. According to the cluster analysis based on UPGMA, the population Gambelas was clearly separated from the other two, which clustered together. The three populations revealed a high level of genetic diversity, with a Nei’s genetic diversity of 0.197 and Shannon’s diversity index of 0.324. A high level of gene flow was observed among populations (Nm ¼ 2.199), reflecting a moderate level of genetic differentiation (GST ¼ 0.185), and no correlation between genetic and geographical distances (r ¼ 1.000;P > 0.05). Based on the results obtained it is concluded that the Gambelas population should be a priority for conservation, including the ex situ propagation and restoration.por
dc.identifier.citationTrindade H., Sena I., Gonçalves S., Romano A. 2012. Genetic diversity of wild populations of Tuberaria major (Cistaceae), an endangered species endemic to the Algarve region (Portugal), using ISSR markers, Biochemical Systematics and Ecology 45: 49–56por
dc.identifier.issn0305-1978
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/2581
dc.language.isoengpor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0305197812001408por
dc.subjectEndemic speciespor
dc.subjectConservation implicationspor
dc.subjectTuberaria majorpor
dc.subjectGenetic structurepor
dc.subjectGenetic diversitypor
dc.subjectISSR markerspor
dc.titleGenetic diversity of wild populations of Tuberaria major (Cistaceae), an endangered species endemic to the Algarve region (Portugal), using ISSR markerspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage56por
oaire.citation.issue1por
oaire.citation.startPage49por
oaire.citation.titleBiochemical Systematics and Ecologypor
oaire.citation.volume45por
person.familyNameGonçalves
person.familyNameRomano
person.givenNameSandra
person.givenNameAnabela
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6988-2017
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa8b335b2-45cc-4578-89b0-153273014280
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscoverya8b335b2-45cc-4578-89b0-153273014280

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