Repository logo
 
Publication

Phenolic composition, antioxidant potential and in vitro inhibitory activity of leaves and acorns of Quercus suber on key enzymes relevant for hyperglycemia and Alzheimer's disease

2015Journal article Restricted access
Simple item page
dc.contributor.authorCustódio, Luísa
dc.contributor.authorPatarra, João
dc.contributor.authorAlbericio, F.
dc.contributor.authorNeng, N. D.
dc.contributor.authorFlorencio Nogueira, J. M. F.
dc.contributor.authorRomano, Anabela
dc.date.accessioned2015-06-18T10:12:34Z
dc.date.available2015-06-18T10:12:34Z
dc.date.issued2015
dc.description.abstractThis work reports the in vitro antioxidant and inhibitory activities of hexane, methanol and water extracts of cork oak (Quercus suber) against acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), a-amylase and a-glucosidase. The total content of phenolics (TPC), tannins (TTC) and flavonoids (TFC), and the HPLC profile of the main phenolic compounds present in the extracts, were also determined. In the 2,2-dipheny1-1-picrylhydrazyl (DPPH) assay the best results were obtained with the methanol and water extracts from both leaves and acorns (41-49%). In the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) method the highest activity was observed with the methanol and leaf water extracts (63-71%). The most significant effects on cholinesterase activity were obtained with the methanol leaf extract at the concentration of 1 mg/mL, yielding inhibition values of 79 and 80% on AChE and BuChE, respectively. No relevant activity was detected against a-amylase, but samples significantly inhibited a-glucosidase from baker's yeast, and the best results were obtained with the water and methanol leaf extracts with values of 97 and 89%, respectively, higher than the positive controls used (acarbose and glucobay). The methanol leaf extract had the highest TPC (211 mg GAE/g, DW) and TFC (8.2 mg RE/g, DW), whereas the water extracts had the highest tannin levels (87 CE/g, DW). The main compound in the methanol leaf extract was gentisic acid (24.3 mg/g, DW). Our results suggest that leaves and acorns from cork oak contain compounds useful for alleviating symptoms associated with Alzheimer disease and other neurodegenerative ailments as well as diabetes. (C) 2014 Elsevier B.V. All rights reserved.
dc.identifier.doihttps://dx.doi.org/10.1016/j.indcrop.2014.11.001
dc.identifier.issn0926-6690
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/6405
dc.language.isoeng
dc.publisherElsevier
dc.relation.isbasedonP-009-ZY1
dc.titlePhenolic composition, antioxidant potential and in vitro inhibitory activity of leaves and acorns of Quercus suber on key enzymes relevant for hyperglycemia and Alzheimer's disease
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage51
oaire.citation.startPage45
oaire.citation.titleIndustrial Crops and Products
oaire.citation.volume64
person.familyNameCustódio
person.familyNameRomano
person.givenNameLuísa
person.givenNameAnabela
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0003-4338-7703
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id15831018900
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationf9cfed0f-6b67-413e-988c-ac7397183471
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscoverye3343b60-17d3-4f5e-a2bf-06f26da69206

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Phenolic composition, antioxidant potential and in vitro inhibitory activity of leaves and acorns of Quercus suber on key enzymes relevant for hyperglycemia and Alzheimer's disease.pdf
Size:
596.46 KB
Format:
Adobe Portable Document Format
Download

Collections

FCT2-Artigos (em revistas ou actas indexadas)