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Temporal metabolic profiling of theQuercus suber-Phytophthora cinnamomisystem by middle-infrared spectroscopy

2015Journal article Open Access
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dc.contributor.authorHardoim, P.R.
dc.contributor.authorGuerra, Rui Manuel Farinha das Neves
dc.contributor.authorCosta, Ana M. Rosa da
dc.contributor.authorSerrano, M. S.
dc.contributor.authorSánchez, M. E.
dc.contributor.authorCoelho, A. C.
dc.date.accessioned2016-01-11T11:14:41Z
dc.date.available2016-01-11T11:14:41Z
dc.date.issued2015
dc.description.abstractThe oomycete Phytophthora cinnamomi is an aggressive plant pathogen, detrimental to many ecosystems including cork oak (Quercus suber) stands, and can inflict great losses in one of the greatest ‘hotspots’ for biodiversity in the world. Here, we applied Fourier transform-infrared (FT-IR) spectroscopy combined with chemometrics to disclose the metabolic patterns of cork oak roots and P. cinnamomi mycelium during the early hours of the interaction. As early as 2 h post-inoculation (hpi), cork oak roots showed altered metabolic patterns with significant variations for regions associated with carbohydrate, glycoconjugate and lipid groups when compared to mockinoculated plants. These variations were further extended at 8 hpi. Surprisingly, at 16 hpi, the metabolic changes in inoculated and mock-inoculated plants were similar, and at 24 hpi, the metabolic patterns of the regions mentioned above were inverted when compared to samples collected at 8 hpi. Principal component analysis of the FT-IR spectra confirmed that the metabolic patterns of inoculated cork oak roots could be readily distinguished from those of mock-inoculated plants at 2, 8 and 24 hpi, but not at 16 hpi. FT-IR spectral analysis from mycelium of P. cinnamomi exposed to cork oak root exudates revealed contrasting variations for regions associated with protein groups at 16 and 24 h post-exposure (hpe), whereas carbohydrate and glycoconjugate groups varied mainly at 24 hpe. Our results revealed early alterations in the metabolic patterns of the host plant when interacting with the biotrophic pathogen. In addition, the FTIR technique can be successfully applied to discriminate infected cork oak plants from mock-inoculated plants, although these differences were dynamic with time. To a lesser extent, the metabolic patterns of P. cinnamomi were also altered when exposed to cork oak root exudates.pt_PT
dc.description.sponsorshipDECOVA (AGL2009-12243-CO2-01)
dc.identifier.citationForest Pathology (in press)pt_PT
dc.identifier.doihttp://dx.doi.org/ 10.1111/efp.12229pt_PT
dc.identifier.otherAUT: RGU01166; AMC01695;
dc.identifier.urihttp://hdl.handle.net/10400.1/7407
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationOMIC METABOLOMICS AND TRANSCRIPTOMICS APPROACHES TO UNRAVEL THE MOLECULAR MECHANISMS OF THE QUERCUS SUBER-PHYTOPHTHORA CINNAMOMI INTERACTION
dc.relationStrategic Project - UI 4023 - 2011-2012
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/efp.12229/epdfpt_PT
dc.subjectQuercus suber–Phytophthorapt_PT
dc.subjectSpectroscopypt_PT
dc.titleTemporal metabolic profiling of theQuercus suber-Phytophthora cinnamomisystem by middle-infrared spectroscopypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleOMIC METABOLOMICS AND TRANSCRIPTOMICS APPROACHES TO UNRAVEL THE MOLECULAR MECHANISMS OF THE QUERCUS SUBER-PHYTOPHTHORA CINNAMOMI INTERACTION
oaire.awardTitleStrategic Project - UI 4023 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F78931%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI4023%2F2011/PT
oaire.citation.titleForest Pathologypt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameHardoim
person.familyNameGuerra
person.familyNameRosa da Costa
person.givenNamePablo
person.givenNameRui
person.givenNameAna M
person.identifier305029
person.identifier.ciencia-id3D16-5067-D6BB
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.orcid0000-0002-5079-4959
person.identifier.orcid0000-0002-8642-5792
person.identifier.orcid0000-0003-0225-9537
person.identifier.ridM-7048-2013
person.identifier.ridE-2165-2012
person.identifier.scopus-author-id24173200900
person.identifier.scopus-author-id53986075100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication41ce75a1-46ff-4d63-84bc-f4b4700e1b46
relation.isAuthorOfPublicationeff7071e-e676-465b-bae3-983f522acd98
relation.isAuthorOfPublication0ca8c16f-6881-4662-bafc-cc51c5ce8d88
relation.isAuthorOfPublication.latestForDiscovery41ce75a1-46ff-4d63-84bc-f4b4700e1b46
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