CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein

Bevelander, G. S.; Pinto, E. S. L. C.; Canario, Adelino V. M.; Spanings, T.; Flik, G.

http://hdl.handle.net/10400.1/3863

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Authors

Bevelander, G. S.

Pinto, E. S. L. C.

Canario, Adelino V. M.

Spanings, T.

Flik, G.

Abstract(s)

Little is known about vitamin D metabolism in fishes. Several reports have shown hydroxylase activities in various organs to produce vitamin D metabolites, but the enzymes involved have not been isolated or characterized. We isolated and characterized a renal mitochondrial hydroxylase, CYP27A1, that governs vitamin D metabolism in gilthead sea bream, Sparus auratus. The enzyme is highly expressed in kidney and to a far lesser extent in liver. When treated with 25-hydroxy vitamin D or calcitriol, the kidney responded differentially and time dependently with CYP27A1 mRNA expression levels. This response substantiates a role for CYP27A1 in fish vitamin D metabolism. This notion is strengthened by upregulation of CYP27A1 in sea bream treated with parathyroid hormone-related protein (PTHrP), and suggests an original role for PTHrP in calcitriol-regulated processes n fish similar to the role of PTH in mammalian vitamin D-dependent processes.

Keywords

D metabolism Fishes

URI

http://hdl.handle.net/10400.1/3863

Citation

Bevelander, G. S; Pinto, E. S L C; Canario, A. V M; Spanings, T.; Flik, G. CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein, Journal of Endocrinology, 196, 3, 625-635, 2008.