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Phase behavior and rheological properties of DNA-cationic polysaccharide mixtures

2012Journal article Restricted access
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dc.contributor.authordos Santos, S.
dc.contributor.authorPiculell, L.
dc.contributor.authorMedronho, B.
dc.contributor.authorMiguel, M. G.
dc.contributor.authorLindman, B.
dc.date.accessioned2015-12-02T14:23:39Z
dc.date.available2015-12-02T14:23:39Z
dc.date.issued2012
dc.description.abstractAssociative aqueous mixtures over a range of concentrations of double- (ds) or single- (ss) stranded DNA with dilute or semidilute solutions of two cationic derivatives of hydroxyethyl cellulose (cat-HEC and cat-HMHEC,(1) the latter carrying grafted hydrophobic groups), were studied. The phase behavior showed an interesting asymmetry: Phase separation occurred immediately when small (sub-stoichiometric) amounts of cationic polyelectrolyte were added to the DNA solution, but redissolution into a single cat-(HM)HEC/DNA/H2O phase occurred already with a modest charge excess of the cationic polyelectrolyte, at a charge ratio approximately independent of the overall polyelectrolyte concentration. Cat-HEC/dsDNA/H2O and cat-HEC/ssDNA/H2O systems presented a considerable difference in the extension of the phase separation region. The one-phase samples with excess cationic polyelectrolyte were studied by rheology. The presence of DNA strengthened the viscoelastic behavior of the solutions of the cationic polyelectrolytes, reflected in an increase in storage modulus and viscosity. Differences in phase behavior and rheology were observed, particularly between systems containing cat-HEC or cat-HMHEC, but also between dsDNA and ssDNA. Thus, these systems allow for the preparation of DNA formulations with widely variable rheology and water uptake. (C) 2012 Elsevier Inc. All rights reserved.
dc.identifier.doihttps://dx.doi.org/10.1016/j.jcis.2012.06.011
dc.identifier.issn0021-9797
dc.identifier.urihttp://hdl.handle.net/10400.1/7262
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.isbasedonP-002-54G
dc.titlePhase behavior and rheological properties of DNA-cationic polysaccharide mixtures
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage74
oaire.citation.startPage63
oaire.citation.titleJournal of collid and interface science
oaire.citation.volume383
person.familyNameMedronho
person.givenNameBruno
person.identifier.ciencia-id6918-9AB3-8D22
person.identifier.orcid0000-0003-0972-1739
person.identifier.ridP-5927-2014
person.identifier.scopus-author-id9940656700
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4c03571a-93da-4504-8acf-c74326449852
relation.isAuthorOfPublication.latestForDiscovery4c03571a-93da-4504-8acf-c74326449852

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