A structured growth model for Cynara cardunculus cell suspension

Cabral, Paula Pires; Lima Costa, Maria Emília; Cabral, J. M. S.

http://hdl.handle.net/10400.1/7583

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Authors

Cabral, Paula Pires

Lima Costa, Maria Emília

Cabral, J. M. S.

Abstract(s)

In this study a model was developed to describe the growth of Cynara cardunculus L. suspended cells as a function of the availability of two substrates, sucrose as the carbon and energy source and phosphate. It was assumed that the maintenance energy need was fulfilled by the consumption of extracellular carbohydrates, in nonlimiting conditions, or by the consumption of structural biomass when sucrose is depleted. A production of secondary metabolites was also assumed. This model was developed based on a structured model previously described by Van Gulik et al. (1993). The model was applied to the experimental results of C. cardunculus suspended cells grown in a Gamborg B-5 medium supplemented with 2% sucrose, using a non-linear regression program.