Publication
Solid phase non isotopic labelling of oligodeoxynucleotides using 5'-protected aminoalkyl phosphoramidites: Application to the specific detection of human papilloma virus DNA
| dc.contributor.author | De Vos, M.J. | |
| dc.contributor.author | Cravador, A. | |
| dc.contributor.author | Lenders, J. P. | |
| dc.contributor.author | Houard, S. | |
| dc.contributor.author | Bollen, A. | |
| dc.date.accessioned | 2015-06-15T09:15:52Z | |
| dc.date.available | 2015-06-15T09:15:52Z | |
| dc.date.issued | 1990 | |
| dc.description.abstract | Phosphoramidites of thymidine or 2'-deoxyinosine, modified in 5' by the addition of an aminoalkylcarbamate function, were prepared. The derivatized nucleotides can be used in automatic DNA synthesis to tag any oligodeoxynucleotide chain and provide a convenient reactive group for labelling with non radioactive reporters. As an example of application, we show the specific detection of Human Papilloma Virus DNA using a biotin-labelled 29-mer oligodeoxynucleotide entirely prepared on solid support. | |
| dc.identifier.issn | 0732-8311 | |
| dc.identifier.other | AUT: ACR00659; | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/6139 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.relation.isbasedon | P-008-R62 | |
| dc.title | Solid phase non isotopic labelling of oligodeoxynucleotides using 5'-protected aminoalkyl phosphoramidites: Application to the specific detection of human papilloma virus DNA | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 273 | |
| oaire.citation.startPage | 259 | |
| oaire.citation.title | Nucleosides and Nucleotides | |
| oaire.citation.volume | 9 | |
| person.familyName | Cravador | |
| person.givenName | Alfredo | |
| person.identifier.orcid | 0000-0002-9831-9815 | |
| person.identifier.rid | K-7247-2012 | |
| person.identifier.scopus-author-id | 6602537257 | |
| rcaap.rights | restrictedAccess | |
| rcaap.type | article | |
| relation.isAuthorOfPublication | 8c84a9e6-6b7f-4b50-93a2-152c85901722 | |
| relation.isAuthorOfPublication.latestForDiscovery | 8c84a9e6-6b7f-4b50-93a2-152c85901722 |
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