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Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata)

2016-09Journal article Restricted access
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dc.contributor.authorde la Serrana, Daniel Garcia
dc.contributor.authorMareco, Edson A.
dc.contributor.authorLA Vieira, Vera
dc.contributor.authorPower, Deborah
dc.contributor.authorJohnston, Ian A.
dc.date.accessioned2017-04-07T15:56:06Z
dc.date.available2017-04-07T15:56:06Z
dc.date.issued2016-09
dc.description.abstractSkeletal muscle, cartilage and bone must function in a co-ordinated fashion during locomotion and growth. In the present study on the gilthead sea bream (Sparus aurata) we tested the hypothesis that muscle and bone differ in their responsiveness to stimuli eliciting fast growth, providing a potential mechanism for generating the skeletal deformities observed in aquaculture. To investigate transcription regulation in skeletal muscle and bone we stimulated protein synthesis using a flooding dose of the branched chain amino acid leucine and compared the results with saline-injected controls. To increase the amount of available sequence information for gene expression analysis a de novo transcriptome was assembled using publicly available Next Generation Sequencing libraries from embryo, fast skeletal muscle, bone and cartilage. The resulting 5 million reads were assembled into 125,646 isotigs representing around 16,000 unique genes, including most components of the Pi3k/Akt/mTor signalling pathway. Principal components analysis was able to distinguish the transcriptional responses between leucine and saline injected controls in skeletal muscle, but not in the bone. General Linear Modelling revealed significant temporal changes in gene expression following leucine injection including the tissue-specific markers sparc, bglap (bone), mlc2 and myod2 (muscle) and gene transcripts associated with Pi3k/Akt/mTor signalling, p70sk6, akt2, ampka and mtor. Skeletal muscle showed more pronounced and rapid changes in transcript abundance than the bone to the same pro-growth signal. The observed differences in transcriptional response are consistent with the idea that fast growth results in a miss-match between muscle and bone development and may contribute to a higher incidence of skeletal deformities. (C) 2016 Elsevier Inc. All rights reserved.
dc.identifier.doihttp://dx.doi.org/10.1016/j.cbpb.2016.02.003
dc.identifier.issn1096-4959
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/9315
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
dc.relationAssociation of European Marine Biological Laboratories
dc.relation.isbasedonWOS:000383820500007
dc.subjectNGS
dc.subjectMuscle
dc.subjectBone
dc.subjectExpression
dc.subjectLeucine
dc.subjectmTOR
dc.subjectFast growth
dc.subjectAquaculture
dc.subjectSkeletal deformities
dc.titleComparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata)
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
oaire.awardTitleAssociation of European Marine Biological Laboratories
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/222719/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/227799/EU
oaire.citation.endPage57
oaire.citation.startPage50
oaire.citation.titleComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology
oaire.citation.volume199
oaire.fundingStreamFP7
oaire.fundingStreamFP7
person.familyNamePower
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isProjectOfPublicationeb95e695-02d8-41f1-88df-951429f0f4f3
relation.isProjectOfPublicationc4772b1a-d59a-4ced-8dc9-943030ba8cfd
relation.isProjectOfPublication.latestForDiscoveryeb95e695-02d8-41f1-88df-951429f0f4f3

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