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Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration

2012Journal article Restricted access
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dc.contributor.authorWhelan, Jessica
dc.contributor.authorMurphy, Eilis
dc.contributor.authorPearson, Alan
dc.contributor.authorJeffers, Paul
dc.contributor.authorKieran, Patricia
dc.contributor.authorMcDonnell, Susan
dc.contributor.authorLima-Costa, Maria Emília
dc.contributor.authorGlennon, Brian
dc.date.accessioned2014-07-23T09:50:06Z
dc.date.available2014-07-23T09:50:06Z
dc.date.issued2012
dc.date.updated2014-07-22T10:09:46Z
dc.description.abstractThe potential of focussed beam reflectance measurement (FBRM) as a tool to monitor changes in biomass concentration was investigated in a number of biological systems. The measurement technique was applied to two morphologically dissimilar plant cell suspension cultures, Morinda citrifolia and Centaurea calcitrapa, to a filamentous bacteria, Streptomyces natalensis, to high density cultures of Escherichia coli and to a murine Sp2/0 hybridoma suspension cell line, 3–2.19. In all cases, the biomass concentration proved to be correlated with total FBRM counts. The nature of the correlation varied between systems and was influenced by the concentration, nature, size and morphology of the particle under investigation.por
dc.identifier.citationWhelan, Jessica; Murphy, Eilis; Pearson, Alan; Jeffers, Paul; Kieran, Patricia; McDonnell, Susan; Lima-Costa, Maria Emília; Glennon, Brian. Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration, Bioprocess and Biosystems Engineering, 35, 6, 963-975, 2012.por
dc.identifier.doihttp://dx.doi.org/ 10.1007/s00449-012-0681-9
dc.identifier.issn1615-7591
dc.identifier.otherAUT: SRA01180;
dc.identifier.urihttp://hdl.handle.net/10400.1/4803
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs00449-012-0681-9por
dc.subjectBiomass concentrationpor
dc.subjectOn-line monitoringpor
dc.subjectFBRMpor
dc.subjectCell morphologypor
dc.titleUse of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentrationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage975por
oaire.citation.issue6por
oaire.citation.startPage963por
oaire.citation.titleBioprocess and Biosystems Engineeringpor
oaire.citation.volume35por
person.familyNameLima Costa
person.givenNameMaria Emília
person.identifier.orcid0000-0002-4433-5756
person.identifier.scopus-author-id7003633118
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36
relation.isAuthorOfPublication.latestForDiscovery6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36

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