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Unraveling the lipidome of Pavlova gyrans, a natural reservoir of bioactive lipids for biotechnological applications

2025-01-02Artigo científico Acesso restrito
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datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorConde, Tiago
dc.contributor.authorLopes, Diana
dc.contributor.authorPinho, Marisa
dc.contributor.authorMelo, Tânia
dc.contributor.authorCoelho, Natacha
dc.contributor.authorRodrigues, Alexandre M. C.
dc.contributor.authorPereira, Hugo
dc.contributor.authorDomingues, Pedro
dc.contributor.authorDomingues, Rosário
dc.date.accessioned2026-04-17T09:15:57Z
dc.date.available2026-04-17T09:15:57Z
dc.date.issued2025-01-02
dc.description.abstractThe high demand for natural bioactive compounds has spurred interest in marine environments as a source of functional ingredients. Pavlova gyrans is a haptophyte microalga characterized by a high amount of omega-3 polyunsaturated fatty acids (PUFA), often found esterified to neutral and polar lipids, the latter possessing nutritional and bioactive properties. However, they are still little investigated in Pavlova species. In this work, we characterized the lipidome of P. gyrans using reverse-phase liquid-chromatography mass spectrometry, the FA profiling by gas chromatography-mass spectrometry, and assessed the anti-inflammatory and antioxidant potential of lipid extracts. The lipid content in P. gyrans was 21.8±1.06 mg (100 mg)−1 biomass, while analysis of the FA profile revealed interesting amounts of eicosapentaenoic (8.1±0.4 mg g−1 biomass) and docosahexaenoic (1.3±0.1 mg g−1 biomass) acids. Lipidomics analysis of P. gyrans allowed the identification of 487 lipid species belonging to different classes of glycolipids (156), phospholipids (46), betaine lipids (111), sphingolipids (67), and neutral lipids (107). Glycolipids accounted for the most abundant lipid classes, highlighting the presence of sulfoquinovosyldiacylglycerol, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol esterified to omega-3 PUFA, some previously reported with bioactive properties. Lipid extracts obtained from P. gyrans revealed a potent anti-inflammatory activity (98.9±0.2%) at 100 µg mL−1, and antioxidant activity with EC50 values of 131.3±3.8 µg mL−1 and 50.8±3.4 µg mL−1 in the scavenging of DPPH and ABTS radicals, respectively. This work highlights P. gyrans as a new source of bioactive polar lipids with promising applications as functional ingredients for the food, feed, nutraceutical, and pharmaceutical industries.eng
dc.identifier.doi10.1007/s10811-024-03420-w
dc.identifier.eissn1573-5176
dc.identifier.issn0921-8971
dc.identifier.urihttp://hdl.handle.net/10400.1/28703
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.ispartofJournal of Applied Phycology
dc.rights.uriN/A
dc.subjectLipidomics
dc.subjectPavlova
dc.subjectHaptophyta
dc.subjectGlycolipids
dc.subjectAnti-inflammatory
dc.subjectAntioxidant
dc.titleUnraveling the lipidome of Pavlova gyrans, a natural reservoir of bioactive lipids for biotechnological applicationseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDP/50017/2020
oaire.awardNumberUIDB/50017/2020
oaire.awardNumberLA/P/0094/2020
oaire.awardNumberUIDB/50006/2020
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0094%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.endPage871
oaire.citation.issue2
oaire.citation.startPage855
oaire.citation.titleJournal of Applied Phycology
oaire.citation.volume37
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePereira
person.givenNameHugo
person.identifier282781
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0002-1369-2099
person.identifier.ridE-9360-2015
person.identifier.scopus-author-id54080259900
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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