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Mitochondrial metabolism of 17α-hydroxyprogesterone in male sea bass (Dicentrarchus labrax): a potential target for endocrine disruptors

2007Journal article Restricted access
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dc.contributor.authorFernandes, Denise
dc.contributor.authorBebianno, Maria João
dc.contributor.authorPorte, Cinta
dc.date.accessioned2020-05-07T14:09:48Z
dc.date.available2020-05-07T14:09:48Z
dc.date.issued2007
dc.description.abstractThe metabolism of 17alpha-hydroxyprogesterone (17P(4)) was investigated in different subcellular fractions isolated from male gonads of sea bass (Dicentrarchus labrax L). The existence of CYP17 (C17,20-lyase activity) and CYP11B (11beta-hydroxylase) catalyzed reactions was demonstrated in the mitochondrial fraction, where 17P(4) was converted to androstenedione (AD) and further metabolized to 11beta-hydroxyandrostenedione (betaAD). The synthesis of betaAD predominated in early spermatogenic testis, indicating a role of betaAD in testicular recrudescence. Additionally, the in vitro effect of model endocrine disrupting chemicals (i.e. nonylphenol (NP), p,p'-DDE, benzo[a]anthracene (BaA), tributyltin (TBT) and ketoconazole (KCZ)) on the mitochondrial metabolism of 17P(4) was investigated. Among the tested compounds, 100 microM NP inhibited the activity of CYP17 (C17,20-lyase) whereas 100 microM KCZ inhibited both CYP17 and CYP11B. Both chemicals showed the potential to disrupt the reproductive cycle of fish living in polluted environments due to impairment of testicular steroid biosynthesis. These results suggest that mitochondrial metabolism of 17P(4) may constitute a new sensitive probe for the assessment of endocrine disruption in fish.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquatox.2007.09.010pt_PT
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.1/13853
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subject17-alpha-hydroxyprogesteronept_PT
dc.subjectAnimalspt_PT
dc.subjectBasspt_PT
dc.subjectBenz(a) anthracenespt_PT
dc.subjectDichlorodiphenyl dichloroethylenept_PT
dc.subjectEndocrine disruptorspt_PT
dc.subjectGas chromatography-mass spectrometrypt_PT
dc.subjectHistocytochemistrypt_PT
dc.subjectMalept_PT
dc.subjectMitochondriapt_PT
dc.subjectPhenolspt_PT
dc.subjectSteroid 11-beta-hydroxylasept_PT
dc.subjectSteroid 17-alpha-hydroxylasept_PT
dc.subjectTestispt_PT
dc.subjectTrialkyltin compoundspt_PT
dc.subjectWater pollutantspt_PT
dc.subjectChemicalpt_PT
dc.titleMitochondrial metabolism of 17α-hydroxyprogesterone in male sea bass (Dicentrarchus labrax): a potential target for endocrine disruptorspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F6123%2F2001/PT
oaire.citation.endPage266pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage258pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume85pt_PT
oaire.fundingStreamSFRH
person.familyNameFernandes
person.familyNameBebianno
person.givenNameDenise
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-0599-3200
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7005622274
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
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