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Divergent responsiveness of the dentary and vertebral bone to a selective estrogen-receptor modulator (SERM) in the teleost Sparus auratus

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dc.contributor.authorVieira, Florbela A.
dc.contributor.authorPinto, Patricia IS
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-24T10:44:41Z
dc.date.available2014-10-24T10:44:41Z
dc.date.issued2012
dc.description.abstractIn teleosts the regulation of skeletal homeostasis and turnover by estrogen is poorly understood. For this reason raloxifene, a selective estrogen-receptor modulator (SERM), was administered to sea bream (Sparus auratus) and its effect on plasma calcium balance and transcript expression in dentary (dermal bone) and vertebra (perichondral bone) was studied. The concentration of total calcium or phosphorus in plasma was unchanged by raloxifene treatment for 6 days. The activity of alkaline phosphatase (ALP) in dentary bone of raloxifene treated fish was significantly (p < 0.05) higher than control fish but it was not changed in vertebral bone. Transcripts for estrogen receptor (ER) a were in very low abundance in the sea bream dentary and vertebra and were unchanged by raloxifene treatment. In contrast, raloxifene caused significant (p < 0.05) up-regulation of the duplicate ERb transcripts in the dentary but did not affect specific transcripts for osteoclast (TRAP), osteoblast (ALP, Runx2, osteonectin) or cartilage (IGF1, CILP2, FN1a). In the vertebra ERbb was not changed by raloxifene but ERba was significantly (p < 0.05) down-regulated as was the skeletal specific transcripts, TRAP, ALP, CILP2, FN1a. In summary, ERbs regulate estrogen sensitivity of the skeleton in sea bream, which responds in a non uniform manner. In common with mammals raloxifene appears to have an anti-resorptive role (in sea bream vertebra), but also an osteoblast stimulatory role, inducing ALP activity in the dentary of sea bream. Overall, the results indicate bone specific responsiveness to raloxifene in the sea bream. Further work will be required to understand the basis of bone responsiveness and the role of E2 and ERs in teleost bone homeostasis.por
dc.description.sponsorshipThe authors would like to acknowledge E. Caldas for her help with the experiment and plasma measurements. This work was funded by the Portuguese National Science Foundation (FCT) grant (POCI/CVT/61052/2004) and a European collective research project (COLL-CT-2005-012451, FINE FISH). F. A. Vieira was funded by FCT (SFRH/BD/17630/2004) and P.I. Pinto was funded by FCT (SFRH/ BPD/25247/2005).por
dc.identifier.citationF.A. Vieira, P.I. Pinto, P.M. Guerreiro, D.M. Power, "Divergent responsiveness of the dentary and vertebral bone to a selective estrogen-receptor modulator (SERM) in the teleost Sparus auratus" in General and Comparative Endocrinology 179 (2012) 421–427.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2012.09.018
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386; PMG01122;
dc.identifier.urihttp://hdl.handle.net/10400.1/5467
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectRaloxifenepor
dc.subjectTeleost bonepor
dc.subjectEstrogen receptorspor
dc.subjectCalcium homeostasispor
dc.titleDivergent responsiveness of the dentary and vertebral bone to a selective estrogen-receptor modulator (SERM) in the teleost Sparus auratuspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage421por
oaire.citation.issue3por
oaire.citation.startPage421por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume179por
person.familyNamePinto
person.familyNameGuerreiro da Costa Guerreiro
person.familyNamePower
person.givenNamePatricia IS
person.givenNamePedro Miguel
person.givenNameDeborah Mary
person.identifier643457
person.identifierA-2539-2009
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscovery64bc526e-5281-44e5-91eb-5743436109f9

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