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Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae

2012Journal article Restricted
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dc.contributor.authorVareilles, Mahaut
dc.contributor.authorRichard, Nadège
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorSilva, Tomé S.
dc.contributor.authorCordeiro, O.
dc.contributor.authorGuerreiro, Inês
dc.contributor.authorYúfera, M.
dc.contributor.authorBatista, I.
dc.contributor.authorPires, C.
dc.contributor.authorPousão-Ferreira, P.
dc.contributor.authorRodrigues, Pedro
dc.contributor.authorRønnestad, I.
dc.contributor.authorFladmark, K. E.
dc.contributor.authorConceição, L. E. C.
dc.date.accessioned2014-06-03T14:42:48Z
dc.date.available2014-06-03T14:42:48Z
dc.date.issued2012
dc.date.updated2014-05-30T09:59:55Z
dc.description.abstractIn order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co-fed from first-feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP-90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in <0.5 kDa hydrolysates). At 15 days after hatching (DAH), proteome expression changes were assessed in entire larvae by twodimensional gel electrophoresis and the quality of larval skeleton was analysed at 28 DAH through double staining of cartilage and bone. Dietary PH fractions tested affected growth, the larvae fed diet L being significantly larger than those fed diet H, but it did not affect the incidence of deformed larvae, nor the number of deformities per fish. Two-dimensional analysis of larvae proteome allowed the detection and the comparative quantification of a total of 709 protein spots having a pI between 4 and 7, around half of which had an expression significantly affected by dietary treatment, the main difference being between proteome of Control larvae with those of both groups L and H. From these spots, 52 proteins involved in diverse processes such as cytoskeletal dynamics, energetic, lipoprotein, amino acid (AA), and nucleotide metabolisms, protein chaperoning and degradation, and signal transduction, were identified. This study revealed that the molecular weight of the dietary protein hydrolysate fraction had a minor impact on skeletal deformities in white seabream larvae, but affected growth performance and had a strong impact on larvae whole body proteome.por
dc.identifier.citationde Vareilles, M.; Richard, N.; Gavaia, P.J.; Silva, T.S.; Cordeiro, O.; Guerreiro, I.; Yúfera, M.; Batista, I.; Pires, C.; Pousão-Ferreira, P.; Rodrigues, P.M.; Rønnestad, I.; Fladmark, K.E.; Conceição, L.E.C. Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae, Journal of Applied Ichthyology, 28, 3, 477-487, 2012.por
dc.identifier.doihttp://dx.doi.org/10.1111/j.1439-0426.2012.01986.x
dc.identifier.issn0175–8659
dc.identifier.urihttp://hdl.handle.net/10400.1/4172
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.titleImpact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvaepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage487por
oaire.citation.issue3por
oaire.citation.startPage477por
oaire.citation.titleJournal of Applied Ichthyologypor
oaire.citation.volume28por
person.familyNameRichard
person.familyNameGavaia
person.familyNameGuerreiro
person.familyNamePousão-Ferreira
person.familyNameRodrigues
person.givenNameNadège
person.givenNamePaulo
person.givenNameInês
person.givenNamePedro
person.givenNamePedro
person.identifierB-6531-2014
person.identifier.ciencia-idB619-FC16-D007
person.identifier.ciencia-idB41A-DD45-85DD
person.identifier.ciencia-id5A11-AB29-0849
person.identifier.orcid0000-0002-8983-0726
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0002-3072-8364
person.identifier.orcid0000-0001-6746-764X
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridM-3385-2013
person.identifier.ridA-6470-2011
person.identifier.ridH-3689-2014
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id15023061500
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id35589829300
person.identifier.scopus-author-id6506349877
person.identifier.scopus-author-id55107531000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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