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Gilthead seabream liver integrative proteomics and metabolomics analysis reveals regulation by different prosurvival pathways in the metabolic adaptation to stress

2022-12-06Journal article Open access
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dc.contributor.authorRaposo de Magalhães, Cláudia
dc.contributor.authorFarinha, Ana Paula
dc.contributor.authorBlackburn, Gavin
dc.contributor.authorWhitfield, Phillip D.
dc.contributor.authorCarrilho, Raquel
dc.contributor.authorSchrama, Denise
dc.contributor.authorCerqueira, Marco
dc.contributor.authorRodrigues, Pedro
dc.date.accessioned2022-12-20T13:23:26Z
dc.date.available2022-12-20T13:23:26Z
dc.date.issued2022-12-06
dc.date.updated2022-12-09T20:23:30Z
dc.description.abstractThe study of the molecular mechanisms of stress appraisal on farmed fish is paramount to ensuring a sustainable aquaculture. Stress exposure can either culminate in the organism’s adaptation or aggravate into a metabolic shutdown, characterized by irreversible cellular damage and deleterious effects on fish performance, welfare, and survival. Multiomics can improve our understanding of the complex stressed phenotype in fish and the molecular mediators that regulate the underlying processes of the molecular stress response. We profiled the stress proteome and metabolome of Sparus aurata responding to different challenges common to aquaculture production, characterizing the disturbed pathways in the fish liver, i.e., the central organ in mounting the stress response. Label-free shotgun proteomics and untargeted metabolomics analyses identified 1738 proteins and 120 metabolites, separately. Mass spectrometry data have been made fully accessible via ProteomeXchange, with the identifier PXD036392, and via MetaboLights, with the identifier MTBLS5940. Integrative multivariate statistical analysis, performed with data integration analysis for biomarker discovery using latent components (DIABLO), depicted the 10 most-relevant features. Functional analysis of these selected features revealed an intricate network of regulatory components, modulating different signaling pathways related to cellular stress, e.g., the mTORC1 pathway, the unfolded protein response, endocytosis, and autophagy to different extents according to the stress nature. These results shed light on the dynamics and extent of this species’ metabolic reprogramming under chronic stress, supporting future studies on stress markers’ discovery and fish welfare research.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInternational Journal of Molecular Sciences 23 (23): 15395 (2022)pt_PT
dc.identifier.doi10.3390/ijms232315395pt_PT
dc.identifier.eissn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.1/18680
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAquaculturept_PT
dc.subjectBioinformaticspt_PT
dc.subjectFish welfarept_PT
dc.subjectHypoxiapt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectMultiomicspt_PT
dc.subjectNet handlingpt_PT
dc.subjectOvercrowdingpt_PT
dc.titleGilthead seabream liver integrative proteomics and metabolomics analysis reveals regulation by different prosurvival pathways in the metabolic adaptation to stresspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue23pt_PT
oaire.citation.startPage15395pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRaposo de Magalhães
person.familyNameMARTINS FARINHA RESENDE
person.familyNameCarrilho
person.familyNameSchrama
person.familyNameCerqueira
person.familyNameRodrigues
person.givenNameCláudia
person.givenNameANA PAULA
person.givenNameRaquel Vaz
person.givenNameDenise
person.givenNameMarco
person.givenNamePedro
person.identifierG-5692-2017
person.identifier159154
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person.identifier.ciencia-idF41E-AC88-8BCD
person.identifier.ciencia-id501C-AC69-68C0
person.identifier.ciencia-id4F1E-16FC-00D8
person.identifier.orcid0000-0001-7751-2870
person.identifier.orcid0000-0003-1455-5958
person.identifier.orcid0000-0003-0274-5957
person.identifier.orcid0000-0001-7237-5053
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridH-6511-2016
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id54386717500
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id55107531000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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