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Genomic structure and expression of parathyroid hormone-related protein gene (PTHrP) in a teleost, Fugu rubripes

2000Journal article Restricted access
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dc.contributor.authorPower, Deborah
dc.contributor.authorIngleton, P. M.
dc.contributor.authorFlanagan, J. A.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorDanks, J. A.
dc.contributor.authorElgar, Greg
dc.contributor.authorClark, M. S.
dc.date.accessioned2014-05-15T13:34:20Z
dc.date.available2014-05-15T13:34:20Z
dc.date.issued2000
dc.date.updated2014-05-13T10:32:33Z
dc.description.abstractIn this study we describe the isolation and characterisation of the parathyroid hormone-related protein (PTHrP) gene from the teleost Fugu rubripes. The gene has a relatively simple structure, compared with tetrapod PTHrP genes, composed of three exons and two introns, encompassing 2.25 kb of genomic DNA. The gene encodes a protein of 163 amino acids, with a putative signal peptide of 37 amino acids and a mature peptide of 126 amino acids. The overall homology with known tetrapod PTHrP proteins is low (36%), with a novel sequence inserted between positions 38 and 65, the absence of the conserved pentapeptide (TRSAW) and shortened C-terminal domain. The N-terminus shows greater conservation (62%), suggesting that it may have a hypercalcaemic function similar to that of tetrapod PTHrP. In situ localisation and RT–PCR have demonstrated the presence of PTHrP in a wide range of tissues with varying levels of expression. Sequence scanning of overlapping cosmids has identified three additional genes, TMPO, LDHB and KCNA1, which map to human chromosome 12, with the latter two mapping to 12p12-11.2. PTHrP in human also maps to this chromosome 12 sub-region, thus demonstrating conservation of synteny between human and Fugu.por
dc.identifier.citationPower, D. M.; Ingleton, P. M.; Flanagan, J. A.; Canario, A. V. M.; Danks, J. A.; Elgar, G.; Clark, M. S.Genomic structure and expression of parathyroid hormone-related protein gene (PTHrP) in a teleost, Fugu rubripes, Gene, 250, 1-2, 67-76, 2000por
dc.identifier.issn0378-111
dc.identifier.otherAUT: DPO00386; ACA00258
dc.identifier.urihttp://hdl.handle.net/10400.1/3961
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectHypercalcaemiapor
dc.subjectVertebratepor
dc.subjectPTHpor
dc.subjectSyntenypor
dc.titleGenomic structure and expression of parathyroid hormone-related protein gene (PTHrP) in a teleost, Fugu rubripespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage76por
oaire.citation.startPage67por
oaire.citation.titleGenepor
oaire.citation.volume250por
person.familyNamePower
person.familyNameCanario
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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