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Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: effects of oral doses of AKG. A proteomic approach

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dc.contributor.authorIbarz, Antoni
dc.contributor.authorCosta, Rita
dc.contributor.authorHarrison, Adrian P.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-21T16:13:14Z
dc.date.available2014-10-21T16:13:14Z
dc.date.issued2010
dc.description.abstractThe influence of a daily oral dose of alpha-ketoglutarate (AKG, 0.1 g/kg body weight), an intermediate metabolite in the Krebs cycle and a dietary additive, on the pituitary proteome of gilthead sea bream was determined by two-dimensional electrophoresis (2-DE). A high-resolution map of the sea bream pituitary proteome was generated. Proteins with a modified expression between Controls and AKG treated fish were further analysed by MALDI-TOF/TOF-MS and liquid chromatography combined with a nanoelectrospray (LC–MS/MS). The main changes in the proteome induced by AKG treatment were grouped. Metabolic proteins up-regulated with AKG supplementation included fructose-bis-phosphate aldolase, glyceraldehyde-phosphate dehydrogenase and malate dehydrogenase, all related to glucose metabolism (p < 0.000). Protein folding related up-regulation with AKG supplementation included two isoforms of heat shock proteins as well as cyclophylin and chaperonin (p < 0.000). An unexpected form of apolipoprotein- A-1 with lower molecular weight (15–16 kDa) was evidenced as being highly abundant in the pituitary proteome of Controls, yet it was down-regulated by AKG treatment. Finally, proteins found to be associated with regeneration of neural function namely cofilin and Vat-protein were up-regulated after AKG supplementation. The only hormone to be modified by AKG treatment was somatolactin, which was significantly down-regulated cf. Controls. In summary, these results provide evidence of a potential endocrine/metabolic regulatory loop activated by AKG supplementation.por
dc.description.sponsorshipThis research was funded by an AQUAGENOME (SSP8, No. 044481) resource exchange grant and pluriannual funding to CCMAR from the Portuguese Science and Technology Foundation (FCT) and XRAq projects (Generalitat de Catalunya, Spain). A.I. was recipient of a research grant from the Spanish Government (Programa de Ayudas de Mobilidad José Castillejo).por
dc.identifier.citationAntoni Ibarz, Rita Costa, Adrian P. Harrison, Deborah M. Power, "Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: Effects of oral doses of AKG. A proteomic approach" in General and Comparative Endocrinology 169 (2010) 284–292.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2010.09.010
dc.identifier.doidoi: 10.1016/j.ygcen.2010.09.010
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5397
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectNeuroendocrinepor
dc.subjectSomatolactinpor
dc.subjectPituitary proteomepor
dc.titleDietary keto-acid feed-back on pituitary activity in gilthead sea bream: effects of oral doses of AKG. A proteomic approachpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage292por
oaire.citation.issue3
oaire.citation.startPage284por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume169por
person.familyNamePower
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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