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Cloning, expression, and tissue localisation of prolactin in adult sea bream (Sparus aurata)

1999Journal article Restricted access
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dc.contributor.authorSantos, Cecilia
dc.contributor.authorBrinca, Lilia
dc.contributor.authorIngleton, P. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-22T13:48:00Z
dc.date.available2014-10-22T13:48:00Z
dc.date.issued1999
dc.description.abstractA major action of prolactin (PRL) in teleost fish is the maintenance of hydromineral balance in euryhaline species in fresh water. The function of PRL in marine teleosts is less certain and unlike euryhaline teleosts, such as tilapia and salmon, there is relatively little information about protein or gene structure. Associated with studies to determine potential functions of PRL, pituitary prolactin cDNA has been cloned and sequenced from sea bream (Sparus aurata), a marine teleost. The sequence obtained spanned 1349 bp and contained an open reading frame encoding a protein of 212 amino acids composed of a putative signal peptide of 24 residues and a mature protein of 188 amino acids. N-terminal sequencing of the native protein confirmed unambiguously the cleavage site, Ala24, Val25, predicted from alignments of the sea bream PRL cDNA with that of other teleosts. The presence of only one form of PRL in sea bream was supported by identification using Northern blots of only a single transcript of 1.35 kb. Reverse transcription and polymerase chain reaction techniques coupled with Southern blot analysis resulted in the detection of PRL in the pituitary but also in the intestine, liver, ovary, and testes.por
dc.description.sponsorshipThis work was supported by a grant from the Portuguese Fundacao para Ciencia e Tecnologia (2/2.1/BIA/211/94). Cecilia R. A. Santos was in receipt of a JNICT grant (BD/2632/93/IG). We are grateful toMs Nair Enxerto for assistance in the study. Note added in proof. In a recent publication Bole-Feysot et al., 1998, refer to over 300 separate functions of PRL in vertebrates. (Bole- Feysot, C., Goffin, V., Edery, M., Binart, N., and Kelly, P. A. (1998). Prolactin (PRL) and its receptor: Actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr. Rev. 19, 225–268).por
dc.identifier.citationC. R. A. Santos, L. Brinca, P. M. Ingleton and D. M. Power. Cloning, Expression, and Tissue Localisation of Prolactin in Adult Sea Bream (Sparus aurata). General and Comparative Endocrinology 114, 57–66 (1999).por
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386; LBR00503;
dc.identifier.urihttp://hdl.handle.net/10400.1/5408
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.titleCloning, expression, and tissue localisation of prolactin in adult sea bream (Sparus aurata)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage66por
oaire.citation.startPage57por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume116por
person.familyNameSantos
person.familyNameBrinca
person.familyNamePower
person.givenNameCecilia
person.givenNameLilia
person.givenNameDeborah Mary
person.identifier.ciencia-id761A-7ED2-D68F
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-6074-7825
person.identifier.orcid0000-0001-9135-8646
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-9032-2016
person.identifier.scopus-author-id7201458751
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication0f8d7c69-8b13-4668-a14b-2d4d6741a6dd
relation.isAuthorOfPublicatione79f75f7-ccb6-46d0-a7d6-54bf8afbefaa
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoverye79f75f7-ccb6-46d0-a7d6-54bf8afbefaa

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