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Cloning, expression, and tissue localisation of prolactin in adult sea bream (Sparus aurata)
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Advisor(s)
Abstract(s)
A major action of prolactin (PRL) in teleost fish is the
maintenance of hydromineral balance in euryhaline species
in fresh water. The function of PRL in marine
teleosts is less certain and unlike euryhaline teleosts,
such as tilapia and salmon, there is relatively little
information about protein or gene structure. Associated
with studies to determine potential functions of PRL,
pituitary prolactin cDNA has been cloned and sequenced
from sea bream (Sparus aurata), a marine teleost. The
sequence obtained spanned 1349 bp and contained an
open reading frame encoding a protein of 212 amino
acids composed of a putative signal peptide of 24
residues and a mature protein of 188 amino acids.
N-terminal sequencing of the native protein confirmed
unambiguously the cleavage site, Ala24, Val25, predicted
from alignments of the sea bream PRL cDNA with that of
other teleosts. The presence of only one form of PRL in
sea bream was supported by identification using Northern
blots of only a single transcript of 1.35 kb. Reverse
transcription and polymerase chain reaction techniques
coupled with Southern blot analysis resulted in the
detection of PRL in the pituitary but also in the intestine,
liver, ovary, and testes.
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Keywords
Citation
C. R. A. Santos, L. Brinca, P. M. Ingleton and D. M. Power. Cloning, Expression, and Tissue Localisation of Prolactin in Adult Sea Bream (Sparus aurata). General and Comparative Endocrinology 114, 57–66 (1999).