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Cartilage acidic protein 1 promotes increased cell viability, cell proliferation and energy metabolism in primary human dermal fibroblasts

2020-04Journal article Restricted access
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dc.contributor.authorLetsiou, Sophia
dc.contributor.authorFélix, Rute
dc.contributor.authorCardoso, João CR
dc.contributor.authorL, Anjos
dc.contributor.authorMestre, Ana L G
dc.contributor.authorH, Gomes
dc.contributor.authorPower, Deborah
dc.date.accessioned2021-06-24T11:35:46Z
dc.date.available2021-06-24T11:35:46Z
dc.date.issued2020-04
dc.description.abstractCartilage acidic protein 1 (CRTAC1) is an extracellular matrix protein of human chondrogenic tissue that is also present in other vertebrates, non-vertebrate eukaryotes and in some prokaryotes. The function of CRTAC1 remains unknown but the protein's structure indicates a role in cell-cell or cell-matrix interactions and calcium-binding. The aim of the present study was to evaluate the in vitro effects of hCRTAC1-A on normal human dermal fibroblasts (NHDF). A battery of in vitro assays (biochemical and PCR), immunofluorescence and a biosensor approach were used to characterize the protein's biological activities on NHDF cells in a scratch assay. Gene expression analysis revealed that hCRTAC1-A protein is associated with altered levels of expression for genes involved in the processes of cell proliferation (CXCL12 and NOS2), cell migration (AQP3 and TNC), and extracellular matrix-ECM regeneration and remodeling (FMOD, TIMP1, FN1) indicating a role for hCRTAC1-A in promoting these activities in a scratch assay. In parallel, the candidate processes identified by differential gene transcription were substantiated and extended using Electric cell-substrate impedance sensing (ECIS) technology, immunofluorescence and cell viability assays. Our findings indicate that hCRTAC1-A stimulated cell proliferation, migration and ECM production in primary human fibroblasts in vitro. (C) 2020 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
dc.description.sponsorshipH2020-MSCA-RISE-2015
dc.description.sponsorshipALGAE4A-B [691102]
dc.description.sponsorshipEMBRIC (European Union's Horizon 2020 research and innovation programme) [654008]
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT)Portuguese Foundation for Science and Technology [UIDB/04326/2020]
dc.description.sponsorshipoperational programme CRESC Algarve 2020 [EMBRC.PT ALG-01-0145-FEDER-022121]
dc.description.sponsorshipoperational programme COMPETE 2020 [EMBRC.PT ALG-01-0145-FEDER-022121]
dc.description.sponsorshipFCTPortuguese Foundation for Science and TechnologyEuropean Commission [UIDB/04326/2020, DL57/2016/CP1361, CT0020, CT0011]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.biochi.2020.02.008
dc.identifier.issn0300-9084
dc.identifier.urihttp://hdl.handle.net/10400.1/16532
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectECIS biosensor
dc.subjectCell migration
dc.subjectCell proliferation
dc.subjecthCRTAC1
dc.subjectIn vitro scratch assay
dc.subjectPrimary human fibroblasts
dc.titleCartilage acidic protein 1 promotes increased cell viability, cell proliferation and energy metabolism in primary human dermal fibroblasts
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage78
oaire.citation.startPage72
oaire.citation.titleBiochimie
oaire.citation.volume171
person.familyNameFélix
person.familyNameCardoso
person.familyNameAnjos Guerreiro
person.familyNameMestre
person.familyNameGomes
person.familyNamePower
person.givenNameRute
person.givenNameJoão
person.givenNameLiliana Isabel Tomé
person.givenNameAna
person.givenNameHenrique Leonel
person.givenNameDeborah Mary
person.identifier14332
person.identifierAAK-3800-2020
person.identifier.ciencia-idFB1A-1325-8E25
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person.identifier.ciencia-id6D18-2D1C-A45C
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person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0002-6679-8698
person.identifier.orcid0000-0003-3664-4740
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-6164-2013
person.identifier.ridM-4151-2013
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id7005305880
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccess
rcaap.typearticle
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