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Crystal structures of the free and sterol-bound forms of beta-cinnamomin

2006Journal article Restricted access
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dc.contributor.authorRodrigues, Maria Luisa
dc.contributor.authorArcher, Margarida
dc.contributor.authorMartel, Paulo
dc.contributor.authorMiranda, Sandra
dc.contributor.authorThomaz, Mónica
dc.contributor.authorEnguita, Francisco J.
dc.contributor.authorBaptista, Ricardo P.
dc.contributor.authorMelo, Eduardo P.
dc.contributor.authorSousa, Nelson
dc.contributor.authorCravador, A.
dc.contributor.authorCarrondo, Maria A.
dc.date.accessioned2012-06-09T11:07:57Z
dc.date.available2012-06-09T11:07:57Z
dc.date.issued2006
dc.date.updated2012-05-20T13:38:54Z
dc.description.abstractThe crystal structure of the elicitin h-cinnamomin (h-CIN) was determined in complex with ergosterol at 1.1 A° resolution. h-CIN/ergosterol complex crystallized in the monoclinic space group P21, with unit cell parameters of a =31.0, b =62.8, c =50.0 A° and b =93.4- and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A° structure of h-CIN (P43212 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of h-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to h-CIN provided an explanation for the apparent inability of h-CIN to bind this ligand, as observed experimentally. D 2005 Elsevier B.V. All rights reserved.por
dc.identifier.citationRodrigues, Maria Luisa; Archer, Margarida; Martel, Paulo; Miranda, Sandra; Thomaz, Mónica; Enguita, Francisco J.; Baptista, Ricardo P.; Melo, Eduardo P.; Sousa, Nelson; Cravador, Alfredo; Carrondo, Maria A. Crystal structures of the free and sterol-bound forms of beta-cinnamomin. Biochimica et Biophysica Acta, 1764, 1, 110-121, 2006.por
dc.identifier.issn1570-9639
dc.identifier.otherAUT: ACR00659; EME01032; PMA01479;
dc.identifier.urihttp://hdl.handle.net/10400.1/1224
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectElicitinpor
dc.subjectSterol carrier proteinpor
dc.subjectCrystal structurepor
dc.subjectPhytophthorapor
dc.titleCrystal structures of the free and sterol-bound forms of beta-cinnamominpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage121por
oaire.citation.issue1764(1)por
oaire.citation.startPage110por
oaire.citation.titleBiochimica et Biophysica Actapor
person.familyNameMartel
person.familyNameCravador
person.givenNamePaulo
person.givenNameAlfredo
person.identifier1955829
person.identifier.ciencia-idAD16-8C2A-26CE
person.identifier.orcid0000-0001-8237-1267
person.identifier.orcid0000-0002-9831-9815
person.identifier.ridK-4704-2013
person.identifier.ridK-7247-2012
person.identifier.scopus-author-id7101625192
person.identifier.scopus-author-id6602537257
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicatione70c3a99-3cde-4b3d-8ca2-4ac252906ae3
relation.isAuthorOfPublication8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isAuthorOfPublication.latestForDiscoverye70c3a99-3cde-4b3d-8ca2-4ac252906ae3

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