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An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant
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Advisor(s)
Abstract(s)
In this study, an efficient protocol was
developed for in vitro propagation of Pinguicula lusitanica L., a rare insectivorous plant with pharmacological
value and limited reproductive capacity. The
effects of two concentrations (0.1 and 0.5 mg l-1) of a range of plant growth regulators, including cytokinins (BA, KIN, and ZEA) and auxins (IAA, IBA, and NAA), and three concentrations of MS medium macronutrients (total, 1/2 and 1/4MS) on proliferation and rooting, were investigated. P. lusitanica shoots
showed abundant proliferation and rooting capacity, both of which were significantly influenced by MS medium strength. The use of 1/2MS supplemented with 0.5 mg l-1 BA or KIN ensure a 29-fold rate of proliferation. Best rooting frequency and higher root number and length were attained in 1/4MS medium containing 0.2 mg l-1 IAA. Sixty percent of the plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development. These plantlets can be used to replenish declining populations in the wild and/or for the extraction of bioactive
compounds, reducing pressure on wild stocks.
Description
Keywords
Conservation In vitro regeneration Plant growth regulators Plantlet production Phytochemicals
Citation
Gonçalves S., Escapa A. L., Grevenstuk T., Romano A. 2008. An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant, Plant Cell, Tissue and Organ Culture 95: 239-243