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An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant

2008Journal article Restricted access
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dc.contributor.authorGonçalves, Sandra
dc.contributor.authorEscapa, Ana Luísa
dc.contributor.authorGrevenstuk, Tomás
dc.contributor.authorRomano, Anabela
dc.date.accessioned2013-03-13T11:57:36Z
dc.date.available2013-03-13T11:57:36Z
dc.date.issued2008
dc.description.abstractIn this study, an efficient protocol was developed for in vitro propagation of Pinguicula lusitanica L., a rare insectivorous plant with pharmacological value and limited reproductive capacity. The effects of two concentrations (0.1 and 0.5 mg l-1) of a range of plant growth regulators, including cytokinins (BA, KIN, and ZEA) and auxins (IAA, IBA, and NAA), and three concentrations of MS medium macronutrients (total, 1/2 and 1/4MS) on proliferation and rooting, were investigated. P. lusitanica shoots showed abundant proliferation and rooting capacity, both of which were significantly influenced by MS medium strength. The use of 1/2MS supplemented with 0.5 mg l-1 BA or KIN ensure a 29-fold rate of proliferation. Best rooting frequency and higher root number and length were attained in 1/4MS medium containing 0.2 mg l-1 IAA. Sixty percent of the plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development. These plantlets can be used to replenish declining populations in the wild and/or for the extraction of bioactive compounds, reducing pressure on wild stocks.por
dc.description.sponsorshipS. Gonçalves and T. Grevenstuk acknowledge a grant from Portuguese Science and Technology Foundation (FCT, Grant SFRH/BPD/31534/2006, Grant SFRH/BD/31777/2006, respectively).por
dc.identifier.citationGonçalves S., Escapa A. L., Grevenstuk T., Romano A. 2008. An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant, Plant Cell, Tissue and Organ Culture 95: 239-243por
dc.identifier.issn0167-6857
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/2559
dc.language.isoengpor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs11240-008-9423-8por
dc.subjectConservationpor
dc.subjectIn vitro regenerationpor
dc.subjectPlant growth regulatorspor
dc.subjectPlantlet productionpor
dc.subjectPhytochemicalspor
dc.titleAn efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plantpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage243por
oaire.citation.issue2por
oaire.citation.startPage239por
oaire.citation.titlePlant Cell Tissue and Organ Culturepor
oaire.citation.volume95por
person.familyNameGonçalves
person.familyNameGrevenstuk
person.familyNameRomano
person.givenNameSandra
person.givenNameTomás
person.givenNameAnabela
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-idB31A-EC35-5A31
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0003-0779-3018
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6988-2017
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id24329026400
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa8b335b2-45cc-4578-89b0-153273014280
relation.isAuthorOfPublication4ae6491f-f21f-4001-8987-dec1ad73ca5c
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscoverya8b335b2-45cc-4578-89b0-153273014280

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