Repository logo
 
Publication

Disruption of thyroid hormone binding to sea bream recombinant transthyretin by ioxinyl and polybrominated diphenyl ethers

2007Journal article Restricted access
Simple item page
dc.contributor.authorMorgado, Isabel
dc.contributor.authorHamers, Timo
dc.contributor.authorVan der Ven, Leo
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-24T10:37:14Z
dc.date.available2014-10-24T10:37:14Z
dc.date.issued2007
dc.description.abstractA number of chemicals released into the environment share structural similarity to the thyroid hormones (THs), thyroxine (T4) and triiodothyronine (T3) and it is thought that they may interfere with the thyroid axis and behave as endocrine disruptors (EDs). One of the ways by which such environmental contaminants may disrupt the TH axis is by binding to TH transporter proteins. Transthyretin (TTR) is one of the thyroid hormone binding proteins responsible for TH transport in the blood. TTR forms a stable tetramer that binds both T4 and T3 and in fish it is principally synthesized in the liver but is also produced by the brain and intestine. In the present study, we investigate the ability of some chemicals arising from pharmaceutical, industrial or agricultural production and classified as EDs, to compete with [I125]-T3 for sea bream recombinant TTR (sbrTTR). Ioxinyl, a common herbicide and several polybrominated diphenyl ethers were strong inhibitors of [I125]-T3 binding to TTR and some showed even greater affinity than the natural ligand T3. The TTR competitive binding assay developed offers a quick and effective tool for preliminary risk assessment of chemicals which may disrupt the thyroid axis in teleost fish inhabiting vulnerable aquatic environments.por
dc.description.sponsorshipThanks to Dr. Marsh and Dr. Ake Bergman of Stockolm University who supplied the BFRs (Table 1) which were synthesised in the context of a EU-supported Project – FIRE (EU Contract No. QLK4-CT-2002-00596; http:// www.rivm.nl/fire>www.rivm.nl/fire). Work co-financed by POCI 2010 and the European social funds attributed by the Portuguese National Science Foundation (FCT) to Project POCTI/CVT/38703/2001, Pluriannual Project to CCMAR and a PhD fellowship to I.M. (SFRH/BD/6091/2001).por
dc.identifier.citationIsabel Morgado, Timo Hamers, Leo Van der Ven, D.M. Power, "Disruption of thyroid hormone binding to sea bream recombinant transthyretin by ioxinyl and polybrominated diphenyl ethers" in Chemosphere 69 (2007) 155–163.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.chemosphere.2007.04.010
dc.identifier.issn0045-6535
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5462
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectLigand binding assaypor
dc.subjectThyroid hormonespor
dc.subjectThyroid hormone binding proteinspor
dc.subjectEndocrine disruptorspor
dc.subjectSea bream transthyretinpor
dc.titleDisruption of thyroid hormone binding to sea bream recombinant transthyretin by ioxinyl and polybrominated diphenyl etherspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage163por
oaire.citation.issue1por
oaire.citation.startPage155por
oaire.citation.titleChemospherepor
oaire.citation.volume69por
person.familyNameMorgado
person.familyNamePower
person.givenNameIsabel
person.givenNameDeborah Mary
person.identifier.ciencia-id3315-7715-C3B4
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-7826-997X
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridJ-4798-2013
person.identifier.scopus-author-id18134268300
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication67026aa2-ccdb-4b68-9938-b09e0fd6aca0
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery67026aa2-ccdb-4b68-9938-b09e0fd6aca0

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Disruption of thyroid hormone binding to sea bream recombinant transthyretin by ioxinyl and polybrominated diphenyl ethers.pdf
Size:
233.43 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.49 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)