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Multivariate cluster analysis to study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts

2008-04Journal article Open Access
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dc.contributor.authorMartínez-Pastor, Felipe
dc.contributor.authorCabrita, Elsa
dc.contributor.authorSoares, Florbela
dc.contributor.authorAnel-Lopez, Luis
dc.contributor.authorDinis, Maria Teresa
dc.date.accessioned2018-12-07T14:52:32Z
dc.date.available2018-12-07T14:52:32Z
dc.date.issued2008-04
dc.description.abstractComputer-assisted sperm analysis (CASA) and clustering analysis have enabled to study sperm subpopulations in mammals, but their use in fish sperm has been limited. We have used spermatozoa. from Senegalese sole (Solea senegalensis) as a model for subpopulation analysis in teleostei using two different activating solutions. Semen from six males was activated using 1100 mOsm/kg solutions: artificial seawater (ASW) or sucrose solution (SUC). Motility was acquired at 15, 30, 45, and 60 s post-activation. CASA parameters were combined into two principal components, which were used in a non-hierarchical clustering analysis, obtaining four subpopulations (CL): CL1 (slow/non-linear), CL2 (slow/linear), CL3 (fast/non-linear), and CL4 (fast/linear). We detected spermatozoa lysis, especially in ASW. Sperm motility was higher for SUC and decreased with time. The subpopulation proportions varied with time and activating treatment, showing both an increase in CL1 and CL2 and a decrease in CO and CL4 with time. Both CL3 and CL4 were higher in samples activated with SUC, at least in early post-activation. Proportions of CO and CL4 at 15 s were associated with higher quality at 60 sand with lower lysis. A second clustering analysis was conducted, classifying the males accordingly to their motility subpopulations. This analysis showed a high heterogeneity between samples. Subpopulation analysis of CASA data can be applied to Solea spermatozoa, allowing identification of potentially interesting sperm subpopulations. Future studies might benefit from these techniques to establish the relationship of these subpopulations with fish sperm quality and fertility, helping to characterize males according to their reproductive potential.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1530/REP-07-0376
dc.identifier.issn1470-1626
dc.identifier.urihttp://hdl.handle.net/10400.1/11109
dc.language.isoeng
dc.peerreviewedyes
dc.publisherBioScientifica
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectIberian red deer
dc.subjectSperm analysis casa
dc.subjectSemen quality
dc.subjectOncorhynchus-mykiss
dc.subjectRainbow-trout
dc.subjectSea-bass
dc.subjectFertilization Success
dc.subjectNucleotide content
dc.subjectFish spermatozoa
dc.subjectEpididymal sperm
dc.titleMultivariate cluster analysis to study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage459
oaire.citation.issue4
oaire.citation.startPage449
oaire.citation.titleReproduction
oaire.citation.volume135
person.familyNameMartínez-Pastor
person.familyNameCabrita
person.familyNameSoares
person.familyNameLuis
person.familyNameDinis
person.givenNameFelipe
person.givenNameElsa
person.givenNameFlorbela
person.givenNameAnel
person.givenNameMaria Teresa
person.identifier.ciencia-id3712-F883-D9F7
person.identifier.ciencia-id5414-4ACF-BF90
person.identifier.orcid0000-0003-2987-4302
person.identifier.orcid0000-0003-3864-8841
person.identifier.orcid0000-0001-6075-2472
person.identifier.orcid0000-0003-2423-4031
person.identifier.orcid0000-0001-7813-1324
person.identifier.ridB-4277-2008
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id8253061600
person.identifier.scopus-author-id26645697000
person.identifier.scopus-author-id35883720000
person.identifier.scopus-author-id55883742900
person.identifier.scopus-author-id7004150905
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication22598537-7c22-4dd7-8220-cb432c2cdc38
relation.isAuthorOfPublication332fa9b9-09b5-47fe-a1b3-afe088b4150a
relation.isAuthorOfPublication51d49c9b-13df-4a40-9b64-90bb12917216
relation.isAuthorOfPublicationd978563a-a2e5-4196-88d0-75c75a68d008
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relation.isAuthorOfPublication.latestForDiscovery309dec19-8aa9-4bea-8665-42dd4b3a9894

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