Repository logo
 
Publication

Proteomics of fish white muscle and Western blotting to detect putative allergens

2022Journal article Open access
Simple item page
dc.contributor.authorAnjos, Liliana
dc.contributor.authorLoukissas, A. Ζ.
dc.contributor.authorPower, Deborah
dc.date.accessioned2023-08-01T10:33:21Z
dc.date.available2023-08-01T10:33:21Z
dc.date.issued2022
dc.description.abstractA detailed workflow is provided for preparation from teleost fish white muscle of extracts for proteomics analysis. The protocol generates samples that can be analyzed by SWATH (Sequential Window data independent Acquisition of the Total High-resolution-Mass Spectra), a modern MS-based quantitative label free technology. The main steps for the extraction of three independent protein fractions, (1) soluble sarcoplasmic, (2) soluble myofibrillar, and (3) insoluble material, from fish white muscle are detailed. Coupled to the protein extraction protocol a Western blotting approach is outlined for detection of common fish allergens, in this case β-parvalbumin, in the white muscle sarcoplasmic protein fraction.pt_PT
dc.description.sponsorshipPO MAR2020, Portugal2020, EU - Fundo Europeu dos Assuntos Marítimos e das Pescas (FEAMP), Project SEAFOODQual (MAR-01.03.01-FEAMP-0050) (Program: MAR2020-P01M03). Additional funds came from H2020-MSCA-RISE-2019, ICHTHYS (n° 872217). FCT provided CCMARs core funding (UIDB/04326/2020) and additional funds came from the operational programs CRESC Algarve 2020, COMPETE 2020, to EMBRC.PT ALG-01-0145-FEDER-022121.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAnjos, L., Loukissas, AZ., Power, D.M. (2022). Proteomics of Fish White Muscle and Western Blotting to Detect Putative Allergens. In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_24pt_PT
dc.identifier.doi10.1007/978-1-0716-2313-8_24pt_PT
dc.identifier.isbn978-1-0716-2313-8
dc.identifier.urihttp://hdl.handle.net/10400.1/19898
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationOptImization of novel value CHains for fish and seafood by developing an integraTed sustainable approacH for improved qualitY, safety and waSte reduction
dc.relationAlgarve Centre for Marine Sciences
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAllergenpt_PT
dc.subjectEuropean sea basspt_PT
dc.subjectProtein extractionpt_PT
dc.subjectProteomicspt_PT
dc.subjectWestern blotpt_PT
dc.subjectβ-parvalbuminpt_PT
dc.titleProteomics of fish white muscle and Western blotting to detect putative allergenspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleOptImization of novel value CHains for fish and seafood by developing an integraTed sustainable approacH for improved qualitY, safety and waSte reduction
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/872217/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0011/PT
oaire.citation.endPage411pt_PT
oaire.citation.startPage397pt_PT
oaire.citation.titleMarine Genomicspt_PT
oaire.citation.volume2498pt_PT
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
person.familyNameAnjos Guerreiro
person.familyNameLOUKISSAS
person.familyNamePower
person.givenNameLiliana Isabel Tomé
person.givenNameARSENIOS ZAFEIRIOS
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0003-3971-5620
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication721b5dca-ad42-40f8-9731-517eb5db009e
relation.isAuthorOfPublication103d2995-45f4-4dbd-95c7-5c5e9fe61922
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery721b5dca-ad42-40f8-9731-517eb5db009e
relation.isProjectOfPublication89c67688-3498-4a31-bcfd-c8142621e215
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication4030cf7e-3a39-47ef-8269-9f56e9ff6089
relation.isProjectOfPublication.latestForDiscovery89c67688-3498-4a31-bcfd-c8142621e215

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Anjos et al Protein extraction Westernblot Final CV revised_Public Acess.pdf
Size:
986.37 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)