Proteomics of fish white muscle and Western blotting to detect putative allergens

Anjos, Liliana; Loukissas, A. Ζ.; Power, Deborah

http://hdl.handle.net/10400.1/19898

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Authors

Anjos, Liliana

Loukissas, A. Ζ.

Power, Deborah

Abstract(s)

A detailed workflow is provided for preparation from teleost fish white muscle of extracts for proteomics analysis. The protocol generates samples that can be analyzed by SWATH (Sequential Window data independent Acquisition of the Total High-resolution-Mass Spectra), a modern MS-based quantitative label free technology. The main steps for the extraction of three independent protein fractions, (1) soluble sarcoplasmic, (2) soluble myofibrillar, and (3) insoluble material, from fish white muscle are detailed. Coupled to the protein extraction protocol a Western blotting approach is outlined for detection of common fish allergens, in this case β-parvalbumin, in the white muscle sarcoplasmic protein fraction.

Keywords

Allergen European sea bass Protein extraction Proteomics Western blot β-parvalbumin

URI

http://hdl.handle.net/10400.1/19898

Citation

Anjos, L., Loukissas, AZ., Power, D.M. (2022). Proteomics of Fish White Muscle and Western Blotting to Detect Putative Allergens. In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_24