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Electrophysiological responses of the clam (Ruditapes decussatus) osphradium to amino acids and alarm cues

2025-09-05Journal article Open access
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dc.contributor.authorRato, Ana Cláudia Nunes
dc.contributor.authorCosta, Joana
dc.contributor.authorGonçalves, Diana
dc.contributor.authorMatias, Domitília
dc.contributor.authorJoaquim, Sandra
dc.contributor.authorHubbard, Peter
dc.date.accessioned2025-09-26T09:38:51Z
dc.date.available2025-09-26T09:38:51Z
dc.date.issued2025-09-05
dc.description.abstractChemical sensing of the surrounding environment is crucial for many aspects of bivalve biology, such as food detection and predator avoidance. Aquatic organisms strongly depend on chemosensory systems; however, little is known about chemosensory systems in bivalves. To understand how the carpet shell clam (Ruditapes decussatus) senses its surrounding chemical environment, we used an electrophysiological technique – the electro-osphradiogram – to assess the sensitivity of the osphradium to different putative odorants (amino acids, bile acids) and odours (predator-released cues and signals from con- and heterospecific bivalves). The clam osphradium was sensitive to most proteinogenic L-amino acids, evoking negative, tonic, and concentration-dependent responses. However, acidic amino acids (L-glutamic and L-aspartic acid), L-arginine and bile acids (cholic, taurocholic and taurolithocholic acid) failed to evoke any response. Surprisingly, while cues from injured bivalves (con- and heterospecific) evoked strong responses, predator-released cues (green crab, Carcinus maenas) failed to elicit any response, whether fed or unfed. That predator-released cues failed to evoke an electrophysiological response in the clam osphradium may indicate that they use cues released by injured prey – alarm cues – to avoid predation and/or that predators are detected by different sensory modalities. Indeed, the behavioural assays, performed to understand how clams make use of such sensory inputs, revealed that the activity index decreased after exposure to water conditioned with injured conspecifics, suggesting the origin of such alarm cues. Further research is needed to identify the chemical nature of these cues. We suggest that the electro-osphradiogram will be a useful tool in this endeavour.eng
dc.identifier.doi10.1007/s00359-025-01757-2
dc.identifier.eissn1432-1351
dc.identifier.issn0340-7594
dc.identifier.urihttp://hdl.handle.net/10400.1/27743
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationSFRH/BD/147215/2019
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationChemical communication in bivalves: hormonal and pheromonal roles
dc.relationOlfactory disruption in the Ria Formosa?
dc.relation.ispartofJournal of Comparative Physiology A
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectElectrophysiology
dc.subjectBivalve
dc.subjectAmino acids
dc.subjectAlarm cues
dc.subjectPredator-released kairomones
dc.subjectOsphradium
dc.titleElectrophysiological responses of the clam (Ruditapes decussatus) osphradium to amino acids and alarm cueseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleChemical communication in bivalves: hormonal and pheromonal roles
oaire.awardTitleOlfactory disruption in the Ria Formosa?
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F147215%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIA-ECO%2F1161%2F2021/PT
oaire.citation.titleJournal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRato
person.familyNameCosta
person.familyNameGonçalves
person.familyNameHubbard
person.givenNameAna Cláudia Nunes
person.givenNameJoana
person.givenNameDiana
person.givenNamePeter
person.identifier.ciencia-id6D14-EA6C-E8D1
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.orcid0000-0002-1150-2757
person.identifier.orcid0009-0005-2098-9818
person.identifier.orcid0000-0001-9107-8484
person.identifier.orcid0000-0002-3007-4647
person.identifier.ridB-2823-2008
person.identifier.scopus-author-id7005928467
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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