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Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway.

2024-12-05Artigo científico Acesso aberto
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dc.contributor.authorLara-Moreno, A.
dc.contributor.authorVargas-Ordóñez, A.
dc.contributor.authorVillaverde, J.
dc.contributor.authorMadrid, F.
dc.contributor.authorCarlier, Jorge
dc.contributor.authorSantos, J. L.
dc.contributor.authorAlonso, E.
dc.contributor.authorMorillo, E.
dc.date.accessioned2024-12-09T11:25:13Z
dc.date.available2024-12-09T11:25:13Z
dc.date.issued2024-12-05
dc.description.abstractWastewater treatment plants (WWTPs) are recognized as significant contributors of paracetamol (APAP) into the environment due to their limited ability to degrade it. This study used a bioaugmentation strategy with Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 to achieve APAP biodegradation in solution in wide ranges of temperature (10-40 °C) and pH (5-9), reaching DT values < 1.5 h to degrade 500 mg L APAP. Bacterial strains also mineralized APAP in solution (<30 %), but when forming consortia with Mycolicibacterium aubagnense HPB1.1, mineralization significantly increased (up to 74 % and 58 % for CSW01 +HPB1.1 and CSW02 +HPB1.1, respectively), decreasing DT values to only 1 and 9 days. Despite the complete degradation of APAP and its high mineralization, residual toxicity throughout the process was observed. Three APAP metabolites were identified (4-aminophenol, hydroquinone and trans-2-hexenoic acid) that quickly disappeared, but residual toxicity remained, indicating the presence of other non-detected intermediates. CSW01 and CSW02 degraded also 100 % APAP (50 mg kg) adsorbed on sewage sludge, with DT values of only 0.7 and 0.3 days, respectively, but < 15 % APAP was mineralized. A genome-based analysis of CSW01 and CSW02 revealed that amidases, deaminases, hydroxylases, and dioxygenases enzymes were involved in APAP biodegradation, and a possible metabolic pathway was proposed.eng
dc.description.sponsorshipJunta de Andalucia (Council of Economic Transformation, Industry, Knowledge and University) - PY20_01069 Margarita Salas grant - European Union's Next Generation EU Junta de Andalucia - AND21_IR-NAS_M2_158
dc.identifier.doi10.1016/j.jhazmat.2024.136128
dc.identifier.other39426148
dc.identifier.urihttp://hdl.handle.net/10400.1/26416
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationBioaugmentation and conjugative plasmid transference using bacteria from extreme environments to enhance biodegradation of recalcitrant pollutants in WWTP granular sludge
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBiodegradation
dc.subjectMineralization
dc.subjectParacetamol
dc.subjectPseudomonas extremaustralis
dc.subjectSewage sludge
dc.subjectStutzerimonas stutzeri
dc.titleBacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway.eng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/CTA-AMB/7782/2020
oaire.awardTitleBioaugmentation and conjugative plasmid transference using bacteria from extreme environments to enhance biodegradation of recalcitrant pollutants in WWTP granular sludge
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTA-AMB%2F7782%2F2020/PT
oaire.citation.startPage136128
oaire.citation.titleJournal of Hazardous Materials
oaire.citation.volume480
oaire.fundingStream3599-PPCDT
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCarlier
person.givenNameJorge
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.orcid0000-0003-0675-2716
person.identifier.scopus-author-id7102166949
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublicationd4af2b00-61d6-4fd9-b05d-9ed235b5c7de
relation.isAuthorOfPublication.latestForDiscoveryd4af2b00-61d6-4fd9-b05d-9ed235b5c7de
relation.isProjectOfPublication0b09c039-bd11-41d7-bebf-ae11435c521d
relation.isProjectOfPublication.latestForDiscovery0b09c039-bd11-41d7-bebf-ae11435c521d

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