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A morphometric characterization of early CHICK embryo elongation

2026-03Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
dc.contributor.authorMaia-Fernandes, Ana C
dc.contributor.authorPais de Azevedo, Tomás
dc.contributor.authorMartins, Nísia Borralho
dc.contributor.authorVentura Ramalhete, Sara Maria
dc.contributor.authorMartins, G. G.
dc.contributor.authorPalmeirim, Isabel
dc.contributor.authordos Santos Duarte, Guilhermina Isabel
dc.contributor.authorMarreiros, Ana
dc.contributor.authorMartel, Paulo
dc.contributor.authorAndrade, Raquel
dc.date.accessioned2026-02-19T13:49:36Z
dc.date.available2026-02-19T13:49:36Z
dc.date.issued2026-03
dc.description.abstractThe chicken embryo has long been a pivotal model system to understand the cellular and molecular mechanisms driving amniote embryo development. Its easy access for in vivo experimentation, together with the development of ex ovo culture techniques, has made it a choice model system for elaborate experimental manipulations. Temporal progression of chick embryo development is classically categorized using the Hamburger and Hamilton staging system (Hamburger, V., & Hamilton, 1951). However, this offers limited temporal resolution when comparing embryos within the same developmental stage and may further be hindered by experimental conditions that directly impact the morphological structures used for stage identification. Here, we performed timelapse imaging of early chick embryonic stages HH4 to HH10 and obtained quantitative elongation data of multiple embryonic portions, yielding two valuable and freely accessible data resources for the chick research community. We identified length measurements capable of describing developmental time, thus enabling the alignment of independent embryos with temporal resolution. Notably, the head-fold (C-HF) showed a strong time correlation, even though it elongates above the primary embryonic axis. A morphometric characterization of HH stages further showed that C-HF length can discriminate HH stages of development, albeit with limited resolution. Finally, we present ChEEQ: Chicken Embryo Elongation Quantification (https://colab.research.google.co m/github/EmbryoClock/ChickElong/blob/main/ChEEQ/ChEEQ.ipynb), a new morphometric tool describing HH4-HH10 embryo elongation, that allows the comparison of user-input data with our reference dataset and is capable of inferring quantitative alterations to embryo developmental time using length measurements alone. Together, these resources open new avenues for investigating vertebrate embryo elongation and quantitatively assessing the effects of experimental interventions on development.eng
dc.description.sponsorshipSFRH/BD/146043/2019; COVID/BD/153553/2024
dc.identifier.doi10.1016/j.ydbio.2025.12.022
dc.identifier.issn0012-1606
dc.identifier.urihttp://hdl.handle.net/10400.1/28196
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationHealth Research Network: From Lab to Community Health
dc.relation.ispartofDevelopmental Biology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectChicken embryo
dc.subjectLength measurements
dc.subjectMorphometric tool
dc.subjectTime-lapse imaging
dc.titleA morphometric characterization of early CHICK embryo elongationeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Research Network: From Lab to Community Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0053%2F2020/PT
oaire.citation.titleDevelopmental biology
oaire.citation.volume531
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameMaia-Fernandes
person.familyNamePais de Azevedo
person.familyNameMartins
person.familyNameVentura Ramalhete
person.familyNamePalmeirim
person.familyNamedos Santos Duarte
person.familyNameMarreiros
person.familyNameMartel
person.familyNameAndrade
person.givenNameAna C
person.givenNameTomás
person.givenNameNísia Borralho
person.givenNameSara Maria
person.givenNameIsabel
person.givenNameGuilhermina Isabel
person.givenNameAna
person.givenNamePaulo
person.givenNameRaquel
person.identifier1921495
person.identifier1955829
person.identifier.ciencia-id1C18-6533-49F5
person.identifier.ciencia-id141A-4DE2-6FF3
person.identifier.ciencia-idED15-7F67-7B64
person.identifier.ciencia-id9A12-9450-7051
person.identifier.ciencia-idAD16-8C2A-26CE
person.identifier.ciencia-id2312-360B-227A
person.identifier.orcid0000-0002-5086-2632
person.identifier.orcid0000-0002-3239-2198
person.identifier.orcid0000-0001-5713-3240
person.identifier.orcid0000-0001-8236-2432
person.identifier.orcid0000-0003-2282-7829
person.identifier.orcid0000-0003-0060-2936
person.identifier.orcid0000-0001-9410-4772
person.identifier.orcid0000-0001-8237-1267
person.identifier.orcid0000-0002-0397-5917
person.identifier.ridK-4704-2013
person.identifier.scopus-author-id55308096300
person.identifier.scopus-author-id6603305227
person.identifier.scopus-author-id57194785077
person.identifier.scopus-author-id7101625192
person.identifier.scopus-author-id7103114918
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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