Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed

Kumar, Praveen Kumar

http://hdl.handle.net/10400.1/10738

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Authors

Kumar, Praveen Kumar

Advisor(s)

Companyó, Ramon

Cavaco, Isabel Maria Palma Antunes ,

Abstract(s)

In this work, clean up strategy is optimized for an analytical method based on liquid chromatography with UV detection for determination of eight sulfonamides (sulfachloropyridazine, sulfadiazine, sulfadimidine, sulfadoxine, sulfamethoxypyridazine, sulfaquinoxaline, sulfamethoxazole and sulfadimethoxine) in pig feed. Solid Phase Extraction is used for clean up and the performance of different sorbents - C 18, Oasis HLB, Plexa and Plexa PCX is assessed by their clean up efficiency and recovery of analytes. Oasis HLB and Plexa PCX sorbents provide better clean up and recovery values than C18 and Plexa. A spiked feed of 2 mg/kg is used to assess mean recoveries and precision in terms of repeatability, r and within-lab reproducibility, R for the whole method with Oasis HLB and Plexa PCX. Recoveries using Oasis HLB ranges from 51 to 78%, with RSDr from 5 to 15% and RSDR from 5 to 18%. Recoveries using Plexa PCX ranges from 64 to 85% with RSDr from 4 to 11% and RSDR from 7 to 17%..