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Chemical profiling and biological screening of Thymus lotocephalus extracts obtained by supercritical fluid extraction and hydrodistillation

2012Journal article Restricted access
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dc.contributor.authorCosta, Patrícia
dc.contributor.authorGonçalves, Sandra
dc.contributor.authorGrosso, Clara
dc.contributor.authorAndrade, Paula B.
dc.contributor.authorValentão, Patrícia
dc.contributor.authorBernardo-Gil, Maria Gabriela
dc.contributor.authorRomano, Anabela
dc.date.accessioned2013-03-15T11:48:56Z
dc.date.available2013-03-15T11:48:56Z
dc.date.issued2012
dc.description.abstractEssential oil and extracts from the aerial parts of Thymus lotocephalus were obtained by hydrodistillation (HD) and supercritical fluid extraction (SFE) in two different collectors, respectively. SFE was conducted at 40 ◦C and a working pressure of 12 or 18 MPa. The chemical profiles were determined using GC-FID and GC–IT–MS. Oxygen-containing monoterpenes were the primary constituents in the essential oil and SFE extracts collected in the second separator, while the extracts obtained in the first separator were predominantly oxygen-containing sesquiterpenes. A large number of compounds were identified by hydrodistillation and, in contrast, the highest extraction yields were obtained using SFE. Linalool(10.43 ± 1.63%) was the main component in essential oil, whereas camphor (7.91 ± 0.84%) and cis-linalool oxide (7.25 ± 1.45%) were the major compounds in the extracts-2nd separator obtained at pressures of 12 and 18 MPa, respectively. Caryophyllene oxide was the primary constituent identified in the extracts-1st separator (4.34 ± 0.51 and 4.41 ± 1.25% obtained at 12 and 18 MPa, respectively). The antioxidant activity was assessed by ORAC and DPPH assays, and the anti-cholinesterase activity was evaluated in vitro using Ellman’s method. The essential oil and SFE extracts (first separator) of T. lotocephalus possessed antioxidant activity and strongly inhibited cholinesterases. We also demonstrated that the acetylcholinesterase and butyrylcholinesterase inhibitory activities of the essential oil could be attributed to 1,8-cineole and caryophyllene oxide, respectively.por
dc.description.sponsorshipP. Costa, C. Grosso and S. Gonçalves acknowledge a grant from the Portuguese Science and Technology Foundation (SFRH/BD/63505/2009, SFRH/BPD/63922/2009 and SFRH/BPD/31534/2006, respectively).por
dc.identifier.citationCosta P., Gonçalves S., Grosso C., Andrade P. B., Valentão P., Bernardo-Gil, M. G., Romano, A. 2012. Chemical profiling and biological screening of Thymus lotocephalus extracts obtained by supercritical fluid extraction and hydrodistillation, Industrial Crops and Products 36: 246–256por
dc.identifier.issn926-6690
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/2586
dc.language.isoengpor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S092666901100389Xpor
dc.subjectEssential oilpor
dc.subjectSFE extractspor
dc.subjectAntioxidantspor
dc.subjectAnti-cholinesterasepor
dc.subjectAlzheimer’s diseasepor
dc.titleChemical profiling and biological screening of Thymus lotocephalus extracts obtained by supercritical fluid extraction and hydrodistillationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage256por
oaire.citation.issue1por
oaire.citation.startPage246por
oaire.citation.titleIndustrial Crops and Productspor
oaire.citation.volume36por
person.familyNameGonçalves
person.familyNameRomano
person.givenNameSandra
person.givenNameAnabela
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6988-2017
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa8b335b2-45cc-4578-89b0-153273014280
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscoverya8b335b2-45cc-4578-89b0-153273014280

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