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Photoperiodic Modulation of Circadian Clock and Reproductive Axis Gene Expression in the Pre-Pubertal European Sea Bass Brain

2015-12Journal article Open access
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dc.contributor.authorMartins, Rute S. T.
dc.contributor.authorGomez, Ana
dc.contributor.authorZanuy, Silvia
dc.contributor.authorCarrillo, Manuel
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2018-12-07T14:52:54Z
dc.date.available2018-12-07T14:52:54Z
dc.date.issued2015-12
dc.description.abstractThe acquisition of reproductive competence requires the activation of the brain-pituitary-gonad (BPG) axis, which in most vertebrates, including fishes, is initiated by changes in photoperiod. In the European sea bass long-term exposure to continuous light (LL) alters the rhythm of reproductive hormones, delays spermatogenesis and reduces the incidence of precocious males. In contrast, an early shift from long to short photoperiod (AP) accelerates spermatogenesis. However, how photoperiod affects key genes in the brain to trigger the onset of puberty is still largely unknown. Here, we investigated if the integration of the light stimulus by clock proteins is sufficient to activate key genes that trigger the BPG axis in the European sea bass. We found that the clock genes clock, npas2, bmal1 and the BPG genes gnrh, kiss and kissr share conserved transcription factor frameworks in their promoters, suggesting co-regulation. Other gene promoters of the BGP axis were also predicted to be co-regulated by the same frameworks. Co-regulation was confirmed through gene expression analysis of brains from males exposed to LL or AP photoperiod compared to natural conditions: LL fish had suppressed gnrh1, kiss2, galr1b and esr1, while AP fish had stimulated npas2, gnrh1, gnrh2, kiss2, kiss1rb and galr1b compared to NP. It is concluded that fish exposed to different photoperiods present significant expression differences in some clock and reproductive axis related genes well before the first detectable endocrine and morphological responses of the BPG axis.
dc.description.sponsorshipEuropean Community [222719 - LIFECYCLE]; Foundation for Science and Technology of Portugal (FCT) [SFRH/BPD/66742/2009, PEst-C/MAR/LA0015/2011]; Valencian Regional Goverment [Prometeo II/2014/051]; Spanish Ministry of Science and Innovation (MICINN) [CSD 2007-0002]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1371/journal.pone.0144158
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/11262
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.relationBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDicentrarchus-Labrax
dc.subjectContinuous light
dc.subjectSexual-maturation
dc.subjectFish
dc.subjectPituitary
dc.subjectL.
dc.subjectGnrh
dc.subjectHormones
dc.subjectSystem
dc.subjectDifferentiation
dc.titlePhotoperiodic Modulation of Circadian Clock and Reproductive Axis Gene Expression in the Pre-Pubertal European Sea Bass Brain
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/222719/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66742%2F2009/PT
oaire.citation.issue12
oaire.citation.startPagee0144158
oaire.citation.titlePLoS ONE
oaire.citation.volume10
oaire.fundingStreamFP7
oaire.fundingStreamSFRH
person.familyNameCanario
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isProjectOfPublicationeb95e695-02d8-41f1-88df-951429f0f4f3
relation.isProjectOfPublication615fba04-a31d-451d-a4d5-398b069bc0d3
relation.isProjectOfPublication.latestForDiscoveryeb95e695-02d8-41f1-88df-951429f0f4f3

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