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Regular supplementation with antioxidants rescues Doxorubicin-Induced Bone deformities and mineralization delay in Zebrafish

2022-11-23Journal article Open access
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dc.contributor.authorPoudel, Sunil
dc.contributor.authorMartins, Gil
dc.contributor.authorCancela, M. Leonor
dc.contributor.authorGavaia, Paulo
dc.date.accessioned2022-12-21T09:38:58Z
dc.date.available2022-12-21T09:38:58Z
dc.date.issued2022-11-23
dc.date.updated2022-12-09T20:23:37Z
dc.description.abstractOsteoporosis is characterized by an abnormal bone structure with low bone mass and degradation of microarchitecture. Oxidative stress induces imbalances in osteoblast and osteoclast activity, leading to bone degradation, a primary cause of secondary osteoporosis. Doxorubicin (DOX) is a widely used chemotherapy drug for treating cancer, known to induce secondary osteoporosis. The mechanism underlying DOX-induced bone loss is still not fully understood, but one of the relevant mechanisms is through a massive accumulation of reactive oxygen and nitrogen species (i.e., ROS and NOS) leading to oxidative stress. We investigated the effects of antioxidants Resveratrol and MitoTEMPO on DOX-induced bone impairment using the zebrafish model. DOX was shown to increase mortality, promote skeletal deformities, induce alterations on intestinal villi, impair growth and mineralization and significantly downregulate osteoblast differentiation markers <i>osteocalcin 2</i> and <i>osterix/sp7</i>. Lipid peroxidation was significantly increased in DOX-supplemented groups as compared to control and antioxidants, suggesting ROS formation as one of the key factors for DOX-induced bone loss. Furthermore, DOX affected mineral contents, suggesting an altered mineral metabolism. However, upon supplementation with antioxidants, DOX-induced effects on mineral content were rescued. Our data show that supplementation with antioxidants effectively improves the overall growth and mineralization in zebrafish and counteracts DOX-induced bone anomalies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNutrients 14 (23): 4959 (2022)pt_PT
dc.identifier.doi10.3390/nu14234959pt_PT
dc.identifier.eissn2072-6643
dc.identifier.urihttp://hdl.handle.net/10400.1/18683
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDoxorubicinpt_PT
dc.subjectMitoTEMPOpt_PT
dc.subjectOxidative stresspt_PT
dc.subjectResveratrolpt_PT
dc.subjectSecondary osteoporosispt_PT
dc.subjectZebrafishpt_PT
dc.titleRegular supplementation with antioxidants rescues Doxorubicin-Induced Bone deformities and mineralization delay in Zebrafishpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue23pt_PT
oaire.citation.startPage4959pt_PT
oaire.citation.titleNutrientspt_PT
oaire.citation.volume14pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePoudel
person.familyNameMartins
person.familyNameCancela
person.familyNameGavaia
person.givenNameSunil
person.givenNameGil
person.givenNameM. Leonor
person.givenNamePaulo
person.identifier.ciencia-id8718-1C0C-CBE8
person.identifier.ciencia-idD11F-8D8A-8EA1
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-3750-0071
person.identifier.orcid0000-0002-1344-2954
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0002-9582-1957
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id6507104377
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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