Publication
Understanding pseudo-albinism in sole (Solea senegalensis): a transcriptomics and metagenomics approach
| dc.contributor.author | Pinto, Patricia IS | |
| dc.contributor.author | Guerreiro, Claudia | |
| dc.contributor.author | Costa, Rita A. | |
| dc.contributor.author | Martinez-Blanch, Juan F. | |
| dc.contributor.author | Carballo, Carlos | |
| dc.contributor.author | Codoñer, Francisco M. | |
| dc.contributor.author | Manchado, Manuel | |
| dc.contributor.author | Power, Deborah | |
| dc.date.accessioned | 2019-10-10T15:37:31Z | |
| dc.date.available | 2019-10-10T15:37:31Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Pseudo-albinism is a pigmentation disorder observed in flatfish aquaculture with a complex, multi-factor aetiology. We tested the hypothesis that pigmentation abnormalities are an overt signal of more generalised modifications in tissue structure and function, using as a model the Senegalese sole and two important innate immune barriers, the skin and intestine, and their microbiomes. Stereological analyses in pseudo-albino sole revealed a significantly increased mucous cell number in skin (P < 0.001) and a significantly thicker muscle layer and lamina propria in gut (P < 0.001). RNA-seq transcriptome analysis of the skin and gut identified 573 differentially expressed transcripts (DETs, FDR < 0.05) between pseudo-albino and pigmented soles (one pool/tissue from 4 individuals/phenotype). DETs were mainly linked to pigment production, skin structure and regeneration and smooth muscle contraction. The microbiome (16 S rRNA analysis) was highly diverse in pigmented and pseudo-albino skin but in gut had low complexity and diverged between the two pigmentation phenotypes. Quantitative PCR revealed significantly lower loads of Mycoplasma (P < 0.05) and Vibrio bacteria (P < 0.01) in pseudo-albino compared to the control. The study revealed that pseudo-albinism in addition to pigmentation changes was associated with generalised changes in the skin and gut structure and a modification in the gut microbiome. | pt_PT |
| dc.description.sponsorship | Agência financiadora H2020 European Funds MSCA-RISE project 691102 Portuguese national funds from FCT - Foundation for Science and Technology UID/Multi/04326/2019 Portuguese national funds from the operational programme CRESC Algarve 2020 EMBRC. PT ALG-01-0145-FEDER-022121 Portuguese national funds from the operational programme COMPETE 2020 EMBRC. PT ALG-01-0145-FEDER-022121 European Union (EU) 654008 Fundacao para a Ciencia e a Tecnologia (FCT) SFRH/BPD/84033/2012 Portuguese Institute for Employment and Vocational Training 0068/ET/18 | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1038/s41598-019-49501-6 | |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/12813 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Nature Publishing Group | pt_PT |
| dc.relation | Development of Microalgae-based novel high added-value products for the Cosmetic and Aquaculture industry | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Understanding pseudo-albinism in sole (Solea senegalensis): a transcriptomics and metagenomics approach | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Development of Microalgae-based novel high added-value products for the Cosmetic and Aquaculture industry | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/691102/EU | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 13604 | pt_PT |
| oaire.citation.title | Scientific Reports | pt_PT |
| oaire.citation.volume | 9 | pt_PT |
| oaire.fundingStream | SFRH | |
| oaire.fundingStream | H2020 | |
| person.familyName | Pinto | |
| person.familyName | guerreiro | |
| person.familyName | Costa | |
| person.familyName | Power | |
| person.givenName | Patricia IS | |
| person.givenName | claudia | |
| person.givenName | Rita | |
| person.givenName | Deborah Mary | |
| person.identifier | 643457 | |
| person.identifier.ciencia-id | E51D-5CCB-B1C6 | |
| person.identifier.ciencia-id | 8219-DFF3-929F | |
| person.identifier.ciencia-id | 891A-8A44-3CAE | |
| person.identifier.orcid | 0000-0001-7854-3898 | |
| person.identifier.orcid | 0000-0003-3585-9563 | |
| person.identifier.orcid | 0000-0002-6975-7576 | |
| person.identifier.orcid | 0000-0003-1366-0246 | |
| person.identifier.rid | M-3817-2013 | |
| person.identifier.rid | O-9136-2018 | |
| person.identifier.scopus-author-id | 10240774300 | |
| person.identifier.scopus-author-id | 57097731700 | |
| person.identifier.scopus-author-id | 7101806760 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | European Commission | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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