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Evaluating the impact of methionine-enriched diets in the liver of European seabass through label-free shotgun proteomics

2021-02Journal article Restricted access
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dc.contributor.authorFarinha, Ana Paula
dc.contributor.authorSchrama, Denise
dc.contributor.authorSilva, Tome
dc.contributor.authorConceicao, Luis E. C.
dc.contributor.authorColen, Rita
dc.contributor.authorEngrola, Sofia
dc.contributor.authorRodrigues, Pedro
dc.contributor.authorCerqueira, Marco
dc.date.accessioned2021-09-08T10:58:07Z
dc.date.available2021-09-08T10:58:07Z
dc.date.issued2021-02
dc.description.abstractPlant protein sources play an essential role in aquaculture by reducing the use of fish meal to sustainable levels, although further supplementation is needed to fulfill fish nutritional requirements. This work addressed fish growth performance and proteome changes to dietary methionine in European seabass juveniles. A dose-dependent response to methionine (Met) was observed on fish growth consistent with proteomic analyses, suggesting Met requirement >= 0.9% (w/w). Fish fed at 0.77% (w/w) exhibited reduced growth and an enrichment in proteins involved in cellular homeostasis. Proteomics data suggest an optimal nutritional status at 1.36% Met (w/w), together with putative beneficial effects on the immune system up to 1.66% Met (w/w). The response to dietary Met involved the convergence of different metabolic and signalling pathways implicated in cell growth and immune response e.g., mTOR, Hedgehog or the T Cell receptor signalling, coupled with a fine-tuning regulation of amino acid metabolism and translation.
dc.description.sponsorshipEuropean Union through FEDER, COMPETE 2020; CRESC Algarve 2020; Portuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal); European Social FundsEuropean Social Fund (ESF) [UIDB/04326/2020, IF/00482/2014/CP1217/CT0005]; [ALG-01-0247-FEDER-3520]
dc.description.sponsorshipALG-01-0247-FEDER-3520
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.jprot.2020.104047
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10400.1/17034
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationAlgarve Centre for Marine Sciences
dc.subjectProteome
dc.subjectAmino acid
dc.subjectNutrition
dc.subjectMetabolism
dc.subjectRegulatory pathway
dc.subjectImmune response
dc.subject.otherBiochemistry & Molecular Biology
dc.titleEvaluating the impact of methionine-enriched diets in the liver of European seabass through label-free shotgun proteomics
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00482%2F2014%2FCP1217%2FCT0005/PT
oaire.citation.startPage104047
oaire.citation.titleJournal of Proteomics
oaire.citation.volume232
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamInvestigador FCT
person.familyNameMARTINS FARINHA RESENDE
person.familyNameSchrama
person.familyNamePontes Barbosa Colen
person.familyNameEngrola
person.familyNameRodrigues
person.familyNameCerqueira
person.givenNameANA PAULA
person.givenNameDenise
person.givenNameRita Isabel
person.givenNameSofia
person.givenNamePedro
person.givenNameMarco
person.identifierG-5692-2017
person.identifier159154
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person.identifier.ciencia-id4312-4B8F-3079
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person.identifier.ciencia-idA610-29E7-9B83
person.identifier.ciencia-id4F1E-16FC-00D8
person.identifier.orcid0000-0003-1455-5958
person.identifier.orcid0000-0003-0274-5957
person.identifier.orcid0000-0001-7104-1844
person.identifier.orcid0000-0002-5244-5541
person.identifier.orcid0000-0002-9668-1204
person.identifier.orcid0000-0001-7237-5053
person.identifier.ridH-6511-2016
person.identifier.ridA-2485-2012
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id54386717500
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id55323905000
person.identifier.scopus-author-id8669620600
person.identifier.scopus-author-id55107531000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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