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Valorization of brewery waste through Polyhydroxyalkanoates production supported by a metabolic specialized microbiome
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Orientador(es)
Resumo(s)
Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large
scale, presents a composition that has been shown to have potential as feedstock for several biological
processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA
production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC)
has not yet been explored. This study explored the feasibility to produce PHA from BSG through the
enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading
rate (OLR) was shown to have only a slight influence on the process performance, although a high
selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on
various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium,
Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome,
but the high diversity in PHA-storing microorganisms could have contributed to the process stability
and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a
yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of
PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock
through the production of added-value products.
Descrição
Palavras-chave
Biopolymers Circular economy Waste valorization Microbial community
Contexto Educativo
Citação
Life 12 (9): 1347 (2022)
Editora
MDPI