Loading...
Publication
Genetic diversity of rotavirus A causing diarrhea in patients admitted to the Clinic University Hospital in Valencia, Spain (2022-2024)
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.77 MB | Adobe PDF |
Authors
Abstract(s)
A diarreia é uma das principais causas de mortalidade infantil em todo o mundo, e o rotavírus destaca-se como o principal agente etiológico associado. Neste contexto, muitos países introduziram a vacina contra o rotavírus no seu calendário de vacinação infantil, incluindo a Espanha. No entanto, a carga das doenças diarreicas continua elevada. Existe uma lacuna de informação em relação a infecção por rotavírus em pacientes que não tenham idade pediátrica e muitos fatores podem estar implicados na suscetibilidade a infeção por rotavírus entre eles os fatores genéticos do hospedeiro denominados Histo Blood Group Antigens (HBGA´s), que podem reconhecer agentes entéricos que modulam doenças entéricas infecciosas, conferindo risco ou suscetibilidade à população. Foi realizada uma análise transversal de dados de base hospitalar, de abril de 2022 a fevereiro de 2024, em 136 pacientes atendidos com diarreia no Hospital Clínico Universitário de Valência. A triagem inicial das amostras foi feita por Real-Time PCR no Hospital Clínico Universitário de Valencia, 136 amostras foram positivas para Rotavírus A (RVA) e testadas por RT-PCR para a identificação do genótipo no laboratório de Microbiologia da Universidade de Valência. A maior diversidade de estirpes de rotavírus foi encontrada em crianças menores de 2 anos e os genótipos mais comuns nesta faixa etária foram G4P[8] e G12P[8]. Cerca de 26,5% das amostras eram não tipificáveis, 16,9% correspondiam a G4P[8], 16,2% eram NTP[8] e 11,8% eram G12P[8]. A sazonalidade foi associada à distribuição das estirpes de rotavírus (p-valor<0,001), com maior pico de infecção em maio, julho e abril de 2023. O status secretor do gene FUT2 foi determinado em 7,7% (2/26) das amostras testadas. A presente análise mostrou uma alta proporção de infecção e diversidade genotípica em crianças com menos de 24 meses de idade. No futuro, será necessário investigar a diversidade genética e a dinâmica evolutiva das estirpes de rotavírus. Embora o estudo tenha encontrado dificuldades na determinação do status secretor para FUT2 a partir das amostras fecais, apresentou informações inovadoras sobre o potencial e as limitações desta abordagem.
Diarrhea is one of the main causes of child mortality worldwide, and rotavirus stands out as the main associated etiological agent. In this context, many countries have introduced the rotavirus vaccine into their childhood vaccination schedule, including Spain. However, the burden of diarrheal diseases remains high. There is a gap in information regarding rotavirus infection in patients other than pediatric age and many factors may be implicated in susceptibility to rotavirus infection, including host genetic factors called Histo Blood Group Antigens (HBGA's), which can recognize enteric agents that modulate infectious enteric diseases, conferring risk or susceptibility to the population. A cross-sectional analysis of hospital-based data was carried out, from April 2022 to February 2024, on 136 patients treated for diarrhea at the University Clinical Hospital of Valencia. The initial screening of samples was carried out by Real-Time PCR at the University Clinical Hospital of Valencia, 136 samples were positive for Rotavirus A (RVA) and tested by RT-PCR to identify the genotype in the Microbiology laboratory of the University of Valencia. The greatest diversity of rotavirus strains was found in children under 2 years of age and the most common genotypes in this age group were G4P[8] and G12P[8]. About 26.5% of the samples were non-typeable, 16.9% corresponded to G4P[8], 16.2% were NTP[8] and 11.8% were G12P[8]. Seasonality was associated with the distribution of rotavirus strains(p-value<0.0001), with the highest peak of infection in May, July and April 2023. The secretory status of the FUT2 gene was determined in 7.7% (2/26) of the samples tested. The present analysis showed a high proportion of infection and genotypic diversity in children under 24 months of age. In the future, it will be necessary to investigate the genetic diversity and evolutionary dynamics of rotavirus strains. Although the study encountered difficulties in determining the secretory status for FUT2 from fecal samples, it presented innovative information about the potential and limitations of this approach.
Diarrhea is one of the main causes of child mortality worldwide, and rotavirus stands out as the main associated etiological agent. In this context, many countries have introduced the rotavirus vaccine into their childhood vaccination schedule, including Spain. However, the burden of diarrheal diseases remains high. There is a gap in information regarding rotavirus infection in patients other than pediatric age and many factors may be implicated in susceptibility to rotavirus infection, including host genetic factors called Histo Blood Group Antigens (HBGA's), which can recognize enteric agents that modulate infectious enteric diseases, conferring risk or susceptibility to the population. A cross-sectional analysis of hospital-based data was carried out, from April 2022 to February 2024, on 136 patients treated for diarrhea at the University Clinical Hospital of Valencia. The initial screening of samples was carried out by Real-Time PCR at the University Clinical Hospital of Valencia, 136 samples were positive for Rotavirus A (RVA) and tested by RT-PCR to identify the genotype in the Microbiology laboratory of the University of Valencia. The greatest diversity of rotavirus strains was found in children under 2 years of age and the most common genotypes in this age group were G4P[8] and G12P[8]. About 26.5% of the samples were non-typeable, 16.9% corresponded to G4P[8], 16.2% were NTP[8] and 11.8% were G12P[8]. Seasonality was associated with the distribution of rotavirus strains(p-value<0.0001), with the highest peak of infection in May, July and April 2023. The secretory status of the FUT2 gene was determined in 7.7% (2/26) of the samples tested. The present analysis showed a high proportion of infection and genotypic diversity in children under 24 months of age. In the future, it will be necessary to investigate the genetic diversity and evolutionary dynamics of rotavirus strains. Although the study encountered difficulties in determining the secretory status for FUT2 from fecal samples, it presented innovative information about the potential and limitations of this approach.
Description
Keywords
Rotavírus Histo blood group antigens Diarreia